EphB2 guides axons at the midline and is necessary for normal vestibular function

Chad A. Cowan, Nobuhiko Yokoyama, Lynne M. Bianchi, Mark Henkemeyer, Bernd Fritzsch

Research output: Contribution to journalArticle

178 Citations (Scopus)

Abstract

Mice lacking the EphB2 receptor tyrosine kinase display a cell-autonomous, strain-specific circling behavior that is associated with vestibular phenotypes. In mutant embryos, the contralateral inner ear efferent growth cones exhibit inappropriate pathway selection at the midline, while in mutant adults, the endolymph-filled lumen of the semicircular canals is severely reduced. EphB2 is expressed in the endolymph-producing dark cells in the inner ear epithelium, and these cells show ultrastructural defects in the mutants. A molecular link to fluid regulation is provided by demonstrating that PDZ domain-containing proteins that bind the C termini of EphB2 and B-ephrins can also recognize the cytoplasmic tails of anion exchangers and aquaporins. This suggests EphB2 may regulate ionic homeostasis and endolymph fluid production through macromolecular associations with membrane channels that transport chloride, bicarbonate, and water.

Original languageEnglish (US)
Pages (from-to)417-430
Number of pages14
JournalNeuron
Volume26
Issue number2
StatePublished - 2000

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Endolymph
EphB2 Receptors
Axons
Inner Ear
Ephrins
PDZ Domains
Semicircular Canals
Growth Cones
Aquaporins
Bicarbonates
Protein C
Ion Channels
Anions
Tail
Chlorides
Homeostasis
Embryonic Structures
Epithelium
Phenotype
Water

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Cowan, C. A., Yokoyama, N., Bianchi, L. M., Henkemeyer, M., & Fritzsch, B. (2000). EphB2 guides axons at the midline and is necessary for normal vestibular function. Neuron, 26(2), 417-430.

EphB2 guides axons at the midline and is necessary for normal vestibular function. / Cowan, Chad A.; Yokoyama, Nobuhiko; Bianchi, Lynne M.; Henkemeyer, Mark; Fritzsch, Bernd.

In: Neuron, Vol. 26, No. 2, 2000, p. 417-430.

Research output: Contribution to journalArticle

Cowan, CA, Yokoyama, N, Bianchi, LM, Henkemeyer, M & Fritzsch, B 2000, 'EphB2 guides axons at the midline and is necessary for normal vestibular function', Neuron, vol. 26, no. 2, pp. 417-430.
Cowan CA, Yokoyama N, Bianchi LM, Henkemeyer M, Fritzsch B. EphB2 guides axons at the midline and is necessary for normal vestibular function. Neuron. 2000;26(2):417-430.
Cowan, Chad A. ; Yokoyama, Nobuhiko ; Bianchi, Lynne M. ; Henkemeyer, Mark ; Fritzsch, Bernd. / EphB2 guides axons at the midline and is necessary for normal vestibular function. In: Neuron. 2000 ; Vol. 26, No. 2. pp. 417-430.
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