Epidermal Growth Factor Receptor-mediated Autocrine and Paracrine Stimulation of Human Transitional Cell Carcinoma

M. E. Gleave, J. T. Hsieh, H. C. Wu, S. J. Hong, H. E. Zhau, P. D. Guthrie, L. W K Chung

Research output: Contribution to journalArticlepeer-review

42 Scopus citations

Abstract

Stromal-epithelial interactions may play a key role in tumor growth and metastasis. We have established a model to study the cellular and molecular basis of this paracrine interaction both in vivo and in vitro using a human transitional cell carcinoma cell line (WH). s.c coinoculation of 1 × 106 WH cells with 1 × 106 nontumorigenic fetal rat urogenital sinus mesenchymal (rUGM) cells in athymic mice accelerated carcinoma growth 20 times faster than isolated WH cell inoculations and 4 times faster than coinoculations of the same number of NIH-3T3 or human bladder fibroblasts. Characterization of these chimeric tumors with im-munohistochemical and DNA dot-blot analyses documented their predominantly human component To evaluate the underlying mechanisms involved in this paracrine-mediated in vivo tumor growth acceleration, Northern analyses for growth factors (GFs) and extracellular matrix (ECM) expression in the different cell lines, as well as in vitro autogenic assays, were performed.

Original languageEnglish (US)
Pages (from-to)5300-5307
Number of pages8
JournalCancer research
Volume53
Issue number21
StatePublished - Nov 1993

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

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