Epidermal growth factor stimulates substrate-selective protein-tyrosine-phosphatase activity

S. M.Teresa Hernández-Sotomayor, Carlos L. Arteaga, Concepció Soler, Graham Carpenter

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

This study investigates the regulation of protein-tyrosine-phosphatase (PTPase; EC 3.1.3.48) activity by epidermal growth factor (EGF). Cytosol from EGF-treated A-431 human epidermoid carcinoma cells was used as a source of PTPase activity, and tyrosine-phosphorylated ErbB2, EGF receptor, phospholipase C-γ1, and the Ras GTPase-activating protein were used as substrates to monitor PTPase activity. EGF stimulated PTPase activity that was selective toward these substrates, as it dephosphorylated ErbB2 and the EGF receptor, but not phospholipase C-γ1 and the Ras GTPase-activating protein. EGF stimulated PTPase activity in several cell lines, regardless of EGF receptor number, and the activity was localized in the cytosol. The dephosphorylation activity in vitro was dependent on the presence of reducing agents and was inhibited by orthovanadate. Agonists such as phorbol 12-myristate 13-acetate, isoproterenol, or ATP were unable to stimulate PTPase activity. Physiological relevance is indicated by experiments showing that EGF treatment of a human mammary cancer cell line rapidly induced the dephosphorylation of ErbB2.

Original languageEnglish (US)
Pages (from-to)7691-7695
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume90
Issue number16
DOIs
StatePublished - Aug 15 1993

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Protein Tyrosine Phosphatases
Epidermal Growth Factor
ras GTPase-Activating Proteins
Epidermal Growth Factor Receptor
Type C Phospholipases
Cytosol
Cell Line
Vanadates
Reducing Agents
Isoproterenol
Tyrosine
Squamous Cell Carcinoma
Acetates
Adenosine Triphosphate
Breast Neoplasms

Keywords

  • Growth factors
  • Phosphotyrosine

ASJC Scopus subject areas

  • General

Cite this

Epidermal growth factor stimulates substrate-selective protein-tyrosine-phosphatase activity. / Hernández-Sotomayor, S. M.Teresa; Arteaga, Carlos L.; Soler, Concepció; Carpenter, Graham.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 90, No. 16, 15.08.1993, p. 7691-7695.

Research output: Contribution to journalArticle

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