Abstract
The purpose of this study was to determine whether epidermal growth factor (EGF), EGF receptor, transforming growth factor alpha (TGF-alpha), transforming growth factor beta (TGF-beta), acidic fibroblast growth factor (acidic-FGF), basic fibroblast growth factor (basic-FCF), and interleukin-1-alpha (IL-1-alpha) proteins were present in cultures of human corneal cells and/or in sections of human corneal tissue. Immunohistochemistry was performed on human corneal sections. Immunofluorescent cell staining was used to evaluate corneal epithelial, stromal fibroblast, and endothelial cells in primary culture. Basic-FGF production was evaluated in culture cells using immunoprecipitation. EGF, TCF-alpha, TGF-beta-1, and IL-1-alpha were detected by immunohistochemistry in cells in all three layers of the cornea. EGF receptor and acidic FCF were detected by immunohistochemistry in epithelial and endothelial cells, but not in stromal fibroblast cells. Differences in distribution of the growth factors were noted within individual layers of the cornea. EGF and basic-FGF proteins were detected in all three predominant cell types of the cornea using immunocytology. IL-1-alpha protein was detected by immunocytology in corneal epithelial and endothelial cells, but not stromal fibroblasts. Immunoprecipitation confirmed the production of basic-FGF in all three cell types. IL-1-alpha protein detection in the corneal stroma by immunohistology, but not by immunocytology in first passage stromal fibroblasts, suggests that IL-1-alpha may localize to the corneal stroma after production by corneal epithelial and/or endothelial cells.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 63-72 |
| Number of pages | 10 |
| Journal | Experimental Eye Research |
| Volume | 59 |
| Issue number | 1 |
| DOIs | |
| State | Published - Jul 1994 |
Fingerprint
Keywords
- acidic fibroblast growth factor
- basic fibroblast growth factor
- cornea
- corneal endothelium
- corneal epithelium
- epidermal growth factor
- interleukin-1-alpha
- keratocytes
- transforming growth factor alpha
- transforming growth factor beta
ASJC Scopus subject areas
- Sensory Systems
- Ophthalmology
Cite this
Epidermal Growth Factor, Transforming Growth Factor Alpha, Transforming Growth Factor Beta, Acidic Fibroblast Growth Factor, Basic Fibroblast Growth Factor, and Interleukin-1 Proteins in the Cornea. / Wilson, Steven E.; Schultz, Gregory S.; Chegini, Nasser; Weng, Jian; He, Yu Guang.
In: Experimental Eye Research, Vol. 59, No. 1, 07.1994, p. 63-72.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Epidermal Growth Factor, Transforming Growth Factor Alpha, Transforming Growth Factor Beta, Acidic Fibroblast Growth Factor, Basic Fibroblast Growth Factor, and Interleukin-1 Proteins in the Cornea
AU - Wilson, Steven E.
AU - Schultz, Gregory S.
AU - Chegini, Nasser
AU - Weng, Jian
AU - He, Yu Guang
PY - 1994/7
Y1 - 1994/7
N2 - The purpose of this study was to determine whether epidermal growth factor (EGF), EGF receptor, transforming growth factor alpha (TGF-alpha), transforming growth factor beta (TGF-beta), acidic fibroblast growth factor (acidic-FGF), basic fibroblast growth factor (basic-FCF), and interleukin-1-alpha (IL-1-alpha) proteins were present in cultures of human corneal cells and/or in sections of human corneal tissue. Immunohistochemistry was performed on human corneal sections. Immunofluorescent cell staining was used to evaluate corneal epithelial, stromal fibroblast, and endothelial cells in primary culture. Basic-FGF production was evaluated in culture cells using immunoprecipitation. EGF, TCF-alpha, TGF-beta-1, and IL-1-alpha were detected by immunohistochemistry in cells in all three layers of the cornea. EGF receptor and acidic FCF were detected by immunohistochemistry in epithelial and endothelial cells, but not in stromal fibroblast cells. Differences in distribution of the growth factors were noted within individual layers of the cornea. EGF and basic-FGF proteins were detected in all three predominant cell types of the cornea using immunocytology. IL-1-alpha protein was detected by immunocytology in corneal epithelial and endothelial cells, but not stromal fibroblasts. Immunoprecipitation confirmed the production of basic-FGF in all three cell types. IL-1-alpha protein detection in the corneal stroma by immunohistology, but not by immunocytology in first passage stromal fibroblasts, suggests that IL-1-alpha may localize to the corneal stroma after production by corneal epithelial and/or endothelial cells.
AB - The purpose of this study was to determine whether epidermal growth factor (EGF), EGF receptor, transforming growth factor alpha (TGF-alpha), transforming growth factor beta (TGF-beta), acidic fibroblast growth factor (acidic-FGF), basic fibroblast growth factor (basic-FCF), and interleukin-1-alpha (IL-1-alpha) proteins were present in cultures of human corneal cells and/or in sections of human corneal tissue. Immunohistochemistry was performed on human corneal sections. Immunofluorescent cell staining was used to evaluate corneal epithelial, stromal fibroblast, and endothelial cells in primary culture. Basic-FGF production was evaluated in culture cells using immunoprecipitation. EGF, TCF-alpha, TGF-beta-1, and IL-1-alpha were detected by immunohistochemistry in cells in all three layers of the cornea. EGF receptor and acidic FCF were detected by immunohistochemistry in epithelial and endothelial cells, but not in stromal fibroblast cells. Differences in distribution of the growth factors were noted within individual layers of the cornea. EGF and basic-FGF proteins were detected in all three predominant cell types of the cornea using immunocytology. IL-1-alpha protein was detected by immunocytology in corneal epithelial and endothelial cells, but not stromal fibroblasts. Immunoprecipitation confirmed the production of basic-FGF in all three cell types. IL-1-alpha protein detection in the corneal stroma by immunohistology, but not by immunocytology in first passage stromal fibroblasts, suggests that IL-1-alpha may localize to the corneal stroma after production by corneal epithelial and/or endothelial cells.
KW - acidic fibroblast growth factor
KW - basic fibroblast growth factor
KW - cornea
KW - corneal endothelium
KW - corneal epithelium
KW - epidermal growth factor
KW - interleukin-1-alpha
KW - keratocytes
KW - transforming growth factor alpha
KW - transforming growth factor beta
UR - http://www.scopus.com/inward/record.url?scp=0028136394&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=0028136394&partnerID=8YFLogxK
U2 - 10.1006/exer.1994.1081
DO - 10.1006/exer.1994.1081
M3 - Article
C2 - 7530663
AN - SCOPUS:0028136394
VL - 59
SP - 63
EP - 72
JO - Experimental Eye Research
JF - Experimental Eye Research
SN - 0014-4835
IS - 1
ER -