Epigenetic Regulation of Coxsackie and Adenovirus Receptor (CAR) Gene Promoter in Urogenital Cancer Cells

Rey Chen Pong, Yun Ju Lai, Hong Chen, Takasugu Okegawa, Eugene P Frenkel, Arthur I Sagalowsky, Jer-Tsong Hsieh

Research output: Contribution to journalArticle

64 Citations (Scopus)

Abstract

Coxsackie and adenovirus receptor (CAR) is not only a high-affinity receptor for adenovirus, but also a tumor inhibitor in both prostate and bladder cancer lines. Decreased CAR gene expression is often detected in cancer specimens; however, the mechanism(s) is still unknown. In this study, we cloned the entire CAR gene and characterized the core promoter sequence of the CAR gene. The CAR gene promoter activity correlated with the differential expression of CAR mRNA levels from several urogenital cancer cell lines, indicating that the down-regulation of CAR gene expression is mediated by transcriptional regulation. It is known that epigenetic control, such as DNA methylation and histone acetylation of chromatin structure, dictates gene expression. Data from this study indicate that the activation of the CAR gene promoter is modulated by histone acetylation, but not by DNA methylation, in urogenital cancer cells. Also, a potent cancer chemotherapeutic agent (FR901228), a histone deacetylase inhibitor, was able to induce endogenous CAR gene expression in several urogenital cancer cells. Taken together, epigenetic control of CAR gene underlies the down-regulation of this gene in urogenital cancers.

Original languageEnglish (US)
Pages (from-to)8680-8686
Number of pages7
JournalCancer Research
Volume63
Issue number24
StatePublished - Dec 15 2003

Fingerprint

Coxsackie and Adenovirus Receptor-Like Membrane Protein
Urogenital Neoplasms
Epigenomics
Genes
Gene Expression
DNA Methylation
Acetylation
Histones
Down-Regulation
Neoplasms
Histone Deacetylase Inhibitors
Urinary Bladder Neoplasms
Chromatin
Prostatic Neoplasms

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Epigenetic Regulation of Coxsackie and Adenovirus Receptor (CAR) Gene Promoter in Urogenital Cancer Cells. / Pong, Rey Chen; Lai, Yun Ju; Chen, Hong; Okegawa, Takasugu; Frenkel, Eugene P; Sagalowsky, Arthur I; Hsieh, Jer-Tsong.

In: Cancer Research, Vol. 63, No. 24, 15.12.2003, p. 8680-8686.

Research output: Contribution to journalArticle

Pong, Rey Chen ; Lai, Yun Ju ; Chen, Hong ; Okegawa, Takasugu ; Frenkel, Eugene P ; Sagalowsky, Arthur I ; Hsieh, Jer-Tsong. / Epigenetic Regulation of Coxsackie and Adenovirus Receptor (CAR) Gene Promoter in Urogenital Cancer Cells. In: Cancer Research. 2003 ; Vol. 63, No. 24. pp. 8680-8686.
@article{10e522f69ad846b5800f2b461cc3d714,
title = "Epigenetic Regulation of Coxsackie and Adenovirus Receptor (CAR) Gene Promoter in Urogenital Cancer Cells",
abstract = "Coxsackie and adenovirus receptor (CAR) is not only a high-affinity receptor for adenovirus, but also a tumor inhibitor in both prostate and bladder cancer lines. Decreased CAR gene expression is often detected in cancer specimens; however, the mechanism(s) is still unknown. In this study, we cloned the entire CAR gene and characterized the core promoter sequence of the CAR gene. The CAR gene promoter activity correlated with the differential expression of CAR mRNA levels from several urogenital cancer cell lines, indicating that the down-regulation of CAR gene expression is mediated by transcriptional regulation. It is known that epigenetic control, such as DNA methylation and histone acetylation of chromatin structure, dictates gene expression. Data from this study indicate that the activation of the CAR gene promoter is modulated by histone acetylation, but not by DNA methylation, in urogenital cancer cells. Also, a potent cancer chemotherapeutic agent (FR901228), a histone deacetylase inhibitor, was able to induce endogenous CAR gene expression in several urogenital cancer cells. Taken together, epigenetic control of CAR gene underlies the down-regulation of this gene in urogenital cancers.",
author = "Pong, {Rey Chen} and Lai, {Yun Ju} and Hong Chen and Takasugu Okegawa and Frenkel, {Eugene P} and Sagalowsky, {Arthur I} and Jer-Tsong Hsieh",
year = "2003",
month = "12",
day = "15",
language = "English (US)",
volume = "63",
pages = "8680--8686",
journal = "Journal of Cancer Research",
issn = "0099-7013",
publisher = "American Association for Cancer Research Inc.",
number = "24",

}

TY - JOUR

T1 - Epigenetic Regulation of Coxsackie and Adenovirus Receptor (CAR) Gene Promoter in Urogenital Cancer Cells

AU - Pong, Rey Chen

AU - Lai, Yun Ju

AU - Chen, Hong

AU - Okegawa, Takasugu

AU - Frenkel, Eugene P

AU - Sagalowsky, Arthur I

AU - Hsieh, Jer-Tsong

PY - 2003/12/15

Y1 - 2003/12/15

N2 - Coxsackie and adenovirus receptor (CAR) is not only a high-affinity receptor for adenovirus, but also a tumor inhibitor in both prostate and bladder cancer lines. Decreased CAR gene expression is often detected in cancer specimens; however, the mechanism(s) is still unknown. In this study, we cloned the entire CAR gene and characterized the core promoter sequence of the CAR gene. The CAR gene promoter activity correlated with the differential expression of CAR mRNA levels from several urogenital cancer cell lines, indicating that the down-regulation of CAR gene expression is mediated by transcriptional regulation. It is known that epigenetic control, such as DNA methylation and histone acetylation of chromatin structure, dictates gene expression. Data from this study indicate that the activation of the CAR gene promoter is modulated by histone acetylation, but not by DNA methylation, in urogenital cancer cells. Also, a potent cancer chemotherapeutic agent (FR901228), a histone deacetylase inhibitor, was able to induce endogenous CAR gene expression in several urogenital cancer cells. Taken together, epigenetic control of CAR gene underlies the down-regulation of this gene in urogenital cancers.

AB - Coxsackie and adenovirus receptor (CAR) is not only a high-affinity receptor for adenovirus, but also a tumor inhibitor in both prostate and bladder cancer lines. Decreased CAR gene expression is often detected in cancer specimens; however, the mechanism(s) is still unknown. In this study, we cloned the entire CAR gene and characterized the core promoter sequence of the CAR gene. The CAR gene promoter activity correlated with the differential expression of CAR mRNA levels from several urogenital cancer cell lines, indicating that the down-regulation of CAR gene expression is mediated by transcriptional regulation. It is known that epigenetic control, such as DNA methylation and histone acetylation of chromatin structure, dictates gene expression. Data from this study indicate that the activation of the CAR gene promoter is modulated by histone acetylation, but not by DNA methylation, in urogenital cancer cells. Also, a potent cancer chemotherapeutic agent (FR901228), a histone deacetylase inhibitor, was able to induce endogenous CAR gene expression in several urogenital cancer cells. Taken together, epigenetic control of CAR gene underlies the down-regulation of this gene in urogenital cancers.

UR - http://www.scopus.com/inward/record.url?scp=0347320795&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0347320795&partnerID=8YFLogxK

M3 - Article

C2 - 14695181

AN - SCOPUS:0347320795

VL - 63

SP - 8680

EP - 8686

JO - Journal of Cancer Research

JF - Journal of Cancer Research

SN - 0099-7013

IS - 24

ER -