Epithelial differentiation with microlumen formation in meningioma: Diagnostic utility of NHERF1/EBP50 immunohistochemistry

Maria Magdalena Georgescu, Adriana Olar, Bret C. Mobley, Phyllis L. Faust, Jack M. Raisanen

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Meningioma is a primary brain tumor arising from the neoplastic transformation of meningothelial cells. Several histological variants of meningioma have been described. Here we show that NHERF1/EBP50, an adaptor protein required for structuring specialized polarized epithelia, can distinguish meningioma variants with epithelial differentiation. NHERF1 decorates the membrane of intracytoplasmic lumens and microlumens in the secretory variant, consistent with a previously described epithelial differentiation of this subtype. NHERF1 also labels microlumens in chordoid meningioma, an epithelial variant not previously known to harbor these structures, and ultrastructural analysis confirmed the presence of microlumens in this variant. NHERF1 associates with the ezrin-radixin-moesin (ERM)-NF2 cytoskeletal proteins, and moesin but not NF2 was detectable in the microlumens. In a meningioma series from 83 patients, NHERF1 revealed microlumens in 87.5% of the chordoid meningioma (n = 25) and meningioma with chordoid component (n = 7) cases, and in 100% of the secretory meningioma cases (n = 12). The most common WHO grade I meningioma variants lacked microlumens. Interestingly, 20% and 66.6% of WHO grades II (n = 20) and III (n = 3) meningiomas, respectively, showed microlumen-like NHERF1 staining of ultrastructural tight microvillar interdigitations, mainly in rhabdoid, papillary-like or sheeting areas, revealing a new subset of high grade meningiomas with epithelial differentiation. NHERF1 failed to detect microlumens in 12 additional cases of chordoid glioma of the 3rd ventricle, chordoma and chondrosarcoma, neoplasms that may mimic the histological appearance of chordoid meningioma. This study uncovers features of epithelial differentiation in meningioma and proposes NHERF1 immunohistochemistry as a method of discriminating chordoid meningioma from neoplasms with similar appearance.

Original languageEnglish (US)
Pages (from-to)28652-28665
Number of pages14
JournalOncotarget
Volume9
Issue number47
DOIs
StatePublished - Jun 19 2018

Fingerprint

Meningioma
Immunohistochemistry
Notochord
Neoplastic Cell Transformation
Chordoma
Chondrosarcoma
Third Ventricle
Cytoskeletal Proteins
Brain Neoplasms
Glioma
Neoplasms
Epithelium

Keywords

  • Chordoid meningioma
  • Epithelial differentiation
  • Microvilli
  • NF2
  • NHERF1/EBP50

ASJC Scopus subject areas

  • Oncology

Cite this

Epithelial differentiation with microlumen formation in meningioma : Diagnostic utility of NHERF1/EBP50 immunohistochemistry. / Georgescu, Maria Magdalena; Olar, Adriana; Mobley, Bret C.; Faust, Phyllis L.; Raisanen, Jack M.

In: Oncotarget, Vol. 9, No. 47, 19.06.2018, p. 28652-28665.

Research output: Contribution to journalArticle

Georgescu, Maria Magdalena ; Olar, Adriana ; Mobley, Bret C. ; Faust, Phyllis L. ; Raisanen, Jack M. / Epithelial differentiation with microlumen formation in meningioma : Diagnostic utility of NHERF1/EBP50 immunohistochemistry. In: Oncotarget. 2018 ; Vol. 9, No. 47. pp. 28652-28665.
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