Epithelial-specific Cre/lox recombination in the developing kidney and genitourinary tract

Xinli Shao, Stefan Somlo, Peter Igarashi

Research output: Contribution to journalArticle

179 Citations (Scopus)

Abstract

Ksp-cadherin is a unique, tissue-specific member of the cadherin family of cell adhesion molecules that is expressed in tubular epithelial cells in the kidney and developing genitourinary (GU) tract. It has recently been shown that a 1341-bp fragment of the 5′ flanking region containing the Ksp-cadherin gene promoter can recapitulate the complete expression pattern of the gene in the developing kidney and GU tract. Similar to the endogenous Ksp-cadherin gene, transgenes containing 1341 bp of the 5′ flanking region are expressed in developing nephrons, ureteric bud, mesonephric tubules, Wolffian duct, and Müllerian duct. In adult mice, the expression is restricted to renal tubules. In the current study, Ksp1.3/Cre transgenic mice carrying 1329 bp of the Kspcadherin 5′ flanking region linked to the Cre recombinase gene were produced. Adult transgenic mice expressed Cre recombinase in renal tubules, especially collecting ducts and thick ascending limbs of Henle's loops. Transgenic embryos expressed Cre recombinase in the branching ureteric bud, developing renal tubules, and sex ducts. Ksp1.3/Cre transgenic mice were crossed with mice carrying a lacZ reporter gene that is activated by Cre/lox-mediated genetic recombination. Bitransgenic progeny expressed lacZ exclusively in renal tubules, mesonephric tubules, ureteric bud, developing ureter, and Wolffian duct. These results demonstrate that Ksp1.3/Cre transgenic mice express Cre recombinase exclusively in the kidney and developing GU tract and can mediate epithelialspecific Cre/lox recombination in these tissues. Ksp1.3/Cre transgenic mice should be useful for cell lineage studies and kidney-specific gene targeting.

Original languageEnglish (US)
Pages (from-to)1837-1846
Number of pages10
JournalJournal of the American Society of Nephrology
Volume13
Issue number7
DOIs
StatePublished - 2002

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Genetic Recombination
Kidney
Transgenic Mice
Cadherins
5' Flanking Region
Wolffian Ducts
Genes
Loop of Henle
Lac Operon
Gene Targeting
Nephrons
Cell Adhesion Molecules
Cell Lineage
Ureter
Transgenes
Reporter Genes
Embryonic Structures
Epithelial Cells
Gene Expression
Cre recombinase

ASJC Scopus subject areas

  • Nephrology

Cite this

Epithelial-specific Cre/lox recombination in the developing kidney and genitourinary tract. / Shao, Xinli; Somlo, Stefan; Igarashi, Peter.

In: Journal of the American Society of Nephrology, Vol. 13, No. 7, 2002, p. 1837-1846.

Research output: Contribution to journalArticle

Shao, Xinli ; Somlo, Stefan ; Igarashi, Peter. / Epithelial-specific Cre/lox recombination in the developing kidney and genitourinary tract. In: Journal of the American Society of Nephrology. 2002 ; Vol. 13, No. 7. pp. 1837-1846.
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