Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues

Yuuki Obata; Shunsuke Kimura; Gaku Nakato; Keito Iizuka; Yurika Miyagawa; Yutaka Nakamura; Yukihiro Furusawa; Machiko Sugiyama; Keiichiro Suzuki; Masashi Ebisawa; Yumiko Fujimura; Hisahiro Yoshida; Toshihiko Iwanaga; Koji Hase; Hiroshi Ohno

doi:10.15252/embr.201438942

Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues

Yuuki Obata, Shunsuke Kimura, Gaku Nakato, Keito Iizuka, Yurika Miyagawa, Yutaka Nakamura, Yukihiro Furusawa, Machiko Sugiyama, Keiichiro Suzuki, Masashi Ebisawa, Yumiko Fujimura, Hisahiro Yoshida, Toshihiko Iwanaga, Koji Hase, Hiroshi Ohno

Research output: Contribution to journal › Article › peer-review

12 Scopus citations

Abstract

Intrinsic Notch signaling in intestinal epithelial cells restricts secretory cell differentiation. In gut-associated lymphoid tissue (GALT), stromal cells located beneath the follicle-associated epithelium (FAE) abundantly express the Notch ligand delta-like 1 (Dll1). Here, we show that mice lacking Rbpj - a gene encoding a transcription factor implicated in Notch signaling - in intestinal epithelial cells have defective GALT maturation. This defect can be attributed to the expansion of goblet cells, which leads to the down-regulation of CCL20 in FAE. These data demonstrate that epithelial Notch signaling maintained by stromal cells contributes to the full maturation of GALT by restricting secretory cell differentiation in FAE.

Original language	English (US)
Pages (from-to)	1297-1304
Number of pages	8
Journal	EMBO Reports
Volume	15
Issue number	12
DOIs	https://doi.org/10.15252/embr.201438942
State	Published - Dec 1 2014
Externally published	Yes

Keywords

Notch signaling
follicle-associated epithelium
gut-associated lymphoid tissues
intestinal epithelial cells

ASJC Scopus subject areas

Biochemistry
Molecular Biology
Genetics

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10.15252/embr.201438942

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Obata, Y., Kimura, S., Nakato, G., Iizuka, K., Miyagawa, Y., Nakamura, Y., Furusawa, Y., Sugiyama, M., Suzuki, K., Ebisawa, M., Fujimura, Y., Yoshida, H., Iwanaga, T., Hase, K., & Ohno, H. (2014). Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues. EMBO Reports, 15(12), 1297-1304. https://doi.org/10.15252/embr.201438942

Obata, Y, Kimura, S, Nakato, G, Iizuka, K, Miyagawa, Y, Nakamura, Y, Furusawa, Y, Sugiyama, M, Suzuki, K, Ebisawa, M, Fujimura, Y, Yoshida, H, Iwanaga, T, Hase, K & Ohno, H 2014, 'Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues', EMBO Reports, vol. 15, no. 12, pp. 1297-1304. https://doi.org/10.15252/embr.201438942

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abstract = "Intrinsic Notch signaling in intestinal epithelial cells restricts secretory cell differentiation. In gut-associated lymphoid tissue (GALT), stromal cells located beneath the follicle-associated epithelium (FAE) abundantly express the Notch ligand delta-like 1 (Dll1). Here, we show that mice lacking Rbpj - a gene encoding a transcription factor implicated in Notch signaling - in intestinal epithelial cells have defective GALT maturation. This defect can be attributed to the expansion of goblet cells, which leads to the down-regulation of CCL20 in FAE. These data demonstrate that epithelial Notch signaling maintained by stromal cells contributes to the full maturation of GALT by restricting secretory cell differentiation in FAE.",

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AU - Iizuka, Keito

AU - Miyagawa, Yurika

AU - Nakamura, Yutaka

AU - Furusawa, Yukihiro

AU - Sugiyama, Machiko

AU - Suzuki, Keiichiro

AU - Ebisawa, Masashi

AU - Fujimura, Yumiko

AU - Yoshida, Hisahiro

AU - Iwanaga, Toshihiko

AU - Hase, Koji

AU - Ohno, Hiroshi

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Epithelial-stromal interaction via Notch signaling is essential for the full maturation of gut-associated lymphoid tissues

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