Epoxyeicosatrienoic acid inhibition alters renal hemodynamics during pregnancy

Hui Huang, Hsin Hsin Chang, Yue Xu, D. Sudarshan Reddy, Juan Du, Yiqiang Zhou, Zheng Dong, J R Falck, Mong Heng Wang

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

In this study we examined the expression of cytochrome P450 (CYP) 2C and CYP2J isoforms in renal proximal tubules and microvessels isolated from rats at different stages of pregnancy. We also selectively inhibited epoxyeicosatrienoic acid (EET) production by the administration of N-methanesulfonyl-6-(2- proparyloxyphenyl)hexanamide (MSPPOH 20 mg/kg/day iv) to rats during Days 14-17 of gestation and to age-matched virgin rats and determined the consequent effects on renal function. Western blot analysis showed that CYP2C11, CYP2C23, and CYP2J2 expression was significantly increased in the renal microvessels of pregnant rats on Day 12 of gestation. In the proximal tubules, CYP2C23 expression was significantly increased throughout pregnancy, while the expression of CYP2C11 was increased in early and late pregnancy and the expression of CYP2J2 was increased in middle and late pregnancy. MSPPOH treatment significantly increased pregnant rats' mean arterial pressure, renal vascular resistance, and sodium balance but significantly decreased renal blood flow, glomerular filtration rate, and urinary sodium excretion, as well as fetal pups' body weight and length. In contrast, MSPPOH treatment had no effect on renal hemodynamics or urinary sodium excretion in age-matched virgin rats. In pregnant rats, MSPPOH treatment also caused selective inhibition of renal cortical EET production and significantly decreased the expression of CYP2C11, CYP2C23, and CYP2J2 in the renal cortex, renal microvessels, and proximal tubules. These results suggest that upregulation of renal vascular and tubular EETs contributes to the control of blood pressure and renal function during pregnancy.

Original languageEnglish (US)
Pages (from-to)1744-1752
Number of pages9
JournalExperimental Biology and Medicine
Volume231
Issue number11
StatePublished - Dec 2006

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Hemodynamics
Rats
Kidney
Pregnancy
Acids
Microvessels
Sodium
Proximal Kidney Tubule
Blood pressure
Fetal Weight
Renal Circulation
Cytochrome P-450 Enzyme System
Glomerular Filtration Rate
Vascular Resistance
Protein Isoforms
Blood
Blood Vessels
Arterial Pressure
Up-Regulation
Western Blotting

Keywords

  • Arachidonic acid
  • CYP2C
  • Cytochrome p450
  • EET
  • Eicosanoids
  • Epoxyeicosatrienoic acid
  • Kidney
  • Pregnancy
  • Renal hemodynamics

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)

Cite this

Huang, H., Chang, H. H., Xu, Y., Reddy, D. S., Du, J., Zhou, Y., ... Wang, M. H. (2006). Epoxyeicosatrienoic acid inhibition alters renal hemodynamics during pregnancy. Experimental Biology and Medicine, 231(11), 1744-1752.

Epoxyeicosatrienoic acid inhibition alters renal hemodynamics during pregnancy. / Huang, Hui; Chang, Hsin Hsin; Xu, Yue; Reddy, D. Sudarshan; Du, Juan; Zhou, Yiqiang; Dong, Zheng; Falck, J R; Wang, Mong Heng.

In: Experimental Biology and Medicine, Vol. 231, No. 11, 12.2006, p. 1744-1752.

Research output: Contribution to journalArticle

Huang, H, Chang, HH, Xu, Y, Reddy, DS, Du, J, Zhou, Y, Dong, Z, Falck, JR & Wang, MH 2006, 'Epoxyeicosatrienoic acid inhibition alters renal hemodynamics during pregnancy', Experimental Biology and Medicine, vol. 231, no. 11, pp. 1744-1752.
Huang H, Chang HH, Xu Y, Reddy DS, Du J, Zhou Y et al. Epoxyeicosatrienoic acid inhibition alters renal hemodynamics during pregnancy. Experimental Biology and Medicine. 2006 Dec;231(11):1744-1752.
Huang, Hui ; Chang, Hsin Hsin ; Xu, Yue ; Reddy, D. Sudarshan ; Du, Juan ; Zhou, Yiqiang ; Dong, Zheng ; Falck, J R ; Wang, Mong Heng. / Epoxyeicosatrienoic acid inhibition alters renal hemodynamics during pregnancy. In: Experimental Biology and Medicine. 2006 ; Vol. 231, No. 11. pp. 1744-1752.
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