Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits

Mirko Messa, Rubén Fernández-Busnadiego, Elizabeth Wen Sun, Hong Chen, Heather Czapla, Kristie Wrasman, Yumei Wu, Genevieve Ko, Theodora Ross, Beverly Wendland, Pietro De Camilli

Research output: Contribution to journalArticle

53 Scopus citations

Abstract

Epsin is an evolutionarily conserved endocytic clathrin adaptor whose most critical function(s) in clathrin coat dynamics remain(s) elusive. To elucidate such function(s), we generated embryonic fibroblasts from conditional epsin triple KO mice. Triple KO cells displayed a dramatic cell division defect. Additionally, a robust impairment in clathrin-mediated endocytosis was observed, with an accumulation of early and U-shaped pits. This defect correlated with a perturbation of the coupling between the clathrin coat and the actin cytoskeleton, which we confirmed in a cell-free assay of endocytosis. Our results indicate that a key evolutionary conserved function of epsin, in addition to other roles that include, as we show here, a low affinity interaction with SNAREs, is to help generate the force that leads to invagination and then fission of clathrin-coated pits.

Original languageEnglish (US)
Article numbere03311
Pages (from-to)1-25
Number of pages25
JournaleLife
Volume3
Issue numberAugust2014
DOIs
StatePublished - Aug 13 2014

ASJC Scopus subject areas

  • Neuroscience(all)
  • Immunology and Microbiology(all)
  • Biochemistry, Genetics and Molecular Biology(all)

Fingerprint Dive into the research topics of 'Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits'. Together they form a unique fingerprint.

  • Cite this

    Messa, M., Fernández-Busnadiego, R., Sun, E. W., Chen, H., Czapla, H., Wrasman, K., Wu, Y., Ko, G., Ross, T., Wendland, B., & De Camilli, P. (2014). Epsin deficiency impairs endocytosis by stalling the actin-dependent invagination of endocytic clathrin-coated pits. eLife, 3(August2014), 1-25. [e03311]. https://doi.org/10.7554/eLife.03311