Epstein-barr virus infection is associated with endothelial Bcl-2 expression in transplant liver allografts

M. T. Millan, Y. Natkunam, R. Clarke-Katzenberg, D. Desai, W. Prapong, S. K. So, C. O. Esquivel, R. Sibley, C. Ferran, O. M. Martinez

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Abstract

Introduction. In liver transplant recipients with Epstein-Barr virus (EBV) disease, we reported a low rate of acute rejection after stopping or markedly lowering immunosuppression. This observation led to the hypothesis that EBV, as a means of viral persistence, induces expression of antiapoptotic factors and these factors, in turn, confer protection to the transplanted organ. Bcl-2, an antiapoptotic factor induced by EBV in various host cells, is not normally expressed in the liver. We questioned whether bcl-2 is expressed in the transplanted liver and whether its expression is modified by EBV. Materials and Methods. Retrospective liver biopsy specimen from liver transplant patients diagnosed with EBV (n=12) were examined for the presence of bcl-2 by immunohistochemistry and compared with EBV (-) transplant (n=15), and nontransplant (n=13) livers. Results. The most significant finding was the presence of endothelial bcl-2 expression in the majority of EBV (+) transplant samples examined (67%) and its relative absence in the other two groups (P<0.005). There was also bcl-2 expression in the hepatocytes and lymphocytes of the majority of transplant liver samples, irrespective of EBV status. Discussion. We have identified a strong association between EBV infection and endothelial bcl-2 expression in transplant livers. We also found that transplantation, in itself, was associated with bcl-2 expression in the hepatocytes and lymphocytes of liver allografts.

Original languageEnglish (US)
Pages (from-to)465-469
Number of pages5
JournalTransplantation
Volume73
Issue number3
StatePublished - Feb 15 2002

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Epstein-Barr Virus Infections
Allografts
Human Herpesvirus 4
Transplants
Liver
Hepatocytes
Lymphocytes
Virus Diseases
Immunosuppression
Transplantation
Immunohistochemistry
Biopsy

ASJC Scopus subject areas

  • Transplantation
  • Immunology

Cite this

Millan, M. T., Natkunam, Y., Clarke-Katzenberg, R., Desai, D., Prapong, W., So, S. K., ... Martinez, O. M. (2002). Epstein-barr virus infection is associated with endothelial Bcl-2 expression in transplant liver allografts. Transplantation, 73(3), 465-469.

Epstein-barr virus infection is associated with endothelial Bcl-2 expression in transplant liver allografts. / Millan, M. T.; Natkunam, Y.; Clarke-Katzenberg, R.; Desai, D.; Prapong, W.; So, S. K.; Esquivel, C. O.; Sibley, R.; Ferran, C.; Martinez, O. M.

In: Transplantation, Vol. 73, No. 3, 15.02.2002, p. 465-469.

Research output: Contribution to journalArticle

Millan, MT, Natkunam, Y, Clarke-Katzenberg, R, Desai, D, Prapong, W, So, SK, Esquivel, CO, Sibley, R, Ferran, C & Martinez, OM 2002, 'Epstein-barr virus infection is associated with endothelial Bcl-2 expression in transplant liver allografts', Transplantation, vol. 73, no. 3, pp. 465-469.
Millan MT, Natkunam Y, Clarke-Katzenberg R, Desai D, Prapong W, So SK et al. Epstein-barr virus infection is associated with endothelial Bcl-2 expression in transplant liver allografts. Transplantation. 2002 Feb 15;73(3):465-469.
Millan, M. T. ; Natkunam, Y. ; Clarke-Katzenberg, R. ; Desai, D. ; Prapong, W. ; So, S. K. ; Esquivel, C. O. ; Sibley, R. ; Ferran, C. ; Martinez, O. M. / Epstein-barr virus infection is associated with endothelial Bcl-2 expression in transplant liver allografts. In: Transplantation. 2002 ; Vol. 73, No. 3. pp. 465-469.
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AU - Millan, M. T.

AU - Natkunam, Y.

AU - Clarke-Katzenberg, R.

AU - Desai, D.

AU - Prapong, W.

AU - So, S. K.

AU - Esquivel, C. O.

AU - Sibley, R.

AU - Ferran, C.

AU - Martinez, O. M.

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N2 - Introduction. In liver transplant recipients with Epstein-Barr virus (EBV) disease, we reported a low rate of acute rejection after stopping or markedly lowering immunosuppression. This observation led to the hypothesis that EBV, as a means of viral persistence, induces expression of antiapoptotic factors and these factors, in turn, confer protection to the transplanted organ. Bcl-2, an antiapoptotic factor induced by EBV in various host cells, is not normally expressed in the liver. We questioned whether bcl-2 is expressed in the transplanted liver and whether its expression is modified by EBV. Materials and Methods. Retrospective liver biopsy specimen from liver transplant patients diagnosed with EBV (n=12) were examined for the presence of bcl-2 by immunohistochemistry and compared with EBV (-) transplant (n=15), and nontransplant (n=13) livers. Results. The most significant finding was the presence of endothelial bcl-2 expression in the majority of EBV (+) transplant samples examined (67%) and its relative absence in the other two groups (P<0.005). There was also bcl-2 expression in the hepatocytes and lymphocytes of the majority of transplant liver samples, irrespective of EBV status. Discussion. We have identified a strong association between EBV infection and endothelial bcl-2 expression in transplant livers. We also found that transplantation, in itself, was associated with bcl-2 expression in the hepatocytes and lymphocytes of liver allografts.

AB - Introduction. In liver transplant recipients with Epstein-Barr virus (EBV) disease, we reported a low rate of acute rejection after stopping or markedly lowering immunosuppression. This observation led to the hypothesis that EBV, as a means of viral persistence, induces expression of antiapoptotic factors and these factors, in turn, confer protection to the transplanted organ. Bcl-2, an antiapoptotic factor induced by EBV in various host cells, is not normally expressed in the liver. We questioned whether bcl-2 is expressed in the transplanted liver and whether its expression is modified by EBV. Materials and Methods. Retrospective liver biopsy specimen from liver transplant patients diagnosed with EBV (n=12) were examined for the presence of bcl-2 by immunohistochemistry and compared with EBV (-) transplant (n=15), and nontransplant (n=13) livers. Results. The most significant finding was the presence of endothelial bcl-2 expression in the majority of EBV (+) transplant samples examined (67%) and its relative absence in the other two groups (P<0.005). There was also bcl-2 expression in the hepatocytes and lymphocytes of the majority of transplant liver samples, irrespective of EBV status. Discussion. We have identified a strong association between EBV infection and endothelial bcl-2 expression in transplant livers. We also found that transplantation, in itself, was associated with bcl-2 expression in the hepatocytes and lymphocytes of liver allografts.

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