ER exit sites are physical and functional core autophagosome biogenesis components

Martin Graef, Jonathan R. Friedman, Christopher Graham, Mohan Babu, Jodi Nunnari

Research output: Contribution to journalArticle

149 Citations (Scopus)

Abstract

Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outstanding questions. Here we show, using proteomic, cytological, and functional analyses, that autophagosomes are spatially, physically, and functionally linked to endoplasmic reticulum exit sites (ERES), which are specialized regions of the endoplasmic reticulum where COPII transport vesicles are generated. Our data demonstrate that ERES are core autophagosomal biogenesis components whose function is required for the hierarchical assembly of the autophagy machinery immediately downstream of the Atg1 kinase complex at phagophore assembly sites.

Original languageEnglish (US)
Pages (from-to)2918-2931
Number of pages14
JournalMolecular biology of the cell
Volume24
Issue number18
DOIs
StatePublished - Sep 15 2013
Externally publishedYes

Fingerprint

Autophagy
Endoplasmic Reticulum
Transport Vesicles
Vacuoles
Lysosomes
Eukaryota
Proteomics
Homeostasis
Phosphotransferases
Membranes
Autophagosomes

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

Cite this

ER exit sites are physical and functional core autophagosome biogenesis components. / Graef, Martin; Friedman, Jonathan R.; Graham, Christopher; Babu, Mohan; Nunnari, Jodi.

In: Molecular biology of the cell, Vol. 24, No. 18, 15.09.2013, p. 2918-2931.

Research output: Contribution to journalArticle

Graef, Martin ; Friedman, Jonathan R. ; Graham, Christopher ; Babu, Mohan ; Nunnari, Jodi. / ER exit sites are physical and functional core autophagosome biogenesis components. In: Molecular biology of the cell. 2013 ; Vol. 24, No. 18. pp. 2918-2931.
@article{947e55074eaa47b5a4e1d0f04815daab,
title = "ER exit sites are physical and functional core autophagosome biogenesis components",
abstract = "Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outstanding questions. Here we show, using proteomic, cytological, and functional analyses, that autophagosomes are spatially, physically, and functionally linked to endoplasmic reticulum exit sites (ERES), which are specialized regions of the endoplasmic reticulum where COPII transport vesicles are generated. Our data demonstrate that ERES are core autophagosomal biogenesis components whose function is required for the hierarchical assembly of the autophagy machinery immediately downstream of the Atg1 kinase complex at phagophore assembly sites.",
author = "Martin Graef and Friedman, {Jonathan R.} and Christopher Graham and Mohan Babu and Jodi Nunnari",
year = "2013",
month = "9",
day = "15",
doi = "10.1091/mbc.E13-07-0381",
language = "English (US)",
volume = "24",
pages = "2918--2931",
journal = "Molecular Biology of the Cell",
issn = "1059-1524",
publisher = "American Society for Cell Biology",
number = "18",

}

TY - JOUR

T1 - ER exit sites are physical and functional core autophagosome biogenesis components

AU - Graef, Martin

AU - Friedman, Jonathan R.

AU - Graham, Christopher

AU - Babu, Mohan

AU - Nunnari, Jodi

PY - 2013/9/15

Y1 - 2013/9/15

N2 - Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outstanding questions. Here we show, using proteomic, cytological, and functional analyses, that autophagosomes are spatially, physically, and functionally linked to endoplasmic reticulum exit sites (ERES), which are specialized regions of the endoplasmic reticulum where COPII transport vesicles are generated. Our data demonstrate that ERES are core autophagosomal biogenesis components whose function is required for the hierarchical assembly of the autophagy machinery immediately downstream of the Atg1 kinase complex at phagophore assembly sites.

AB - Autophagy is a central homeostasis and stress response pathway conserved in all eukaryotes. One hallmark of autophagy is the de novo formation of autophagosomes. These double-membrane vesicular structures form around and deliver cargo for degradation by the vacuole/lysosome. Where and how autophagosomes form are outstanding questions. Here we show, using proteomic, cytological, and functional analyses, that autophagosomes are spatially, physically, and functionally linked to endoplasmic reticulum exit sites (ERES), which are specialized regions of the endoplasmic reticulum where COPII transport vesicles are generated. Our data demonstrate that ERES are core autophagosomal biogenesis components whose function is required for the hierarchical assembly of the autophagy machinery immediately downstream of the Atg1 kinase complex at phagophore assembly sites.

UR - http://www.scopus.com/inward/record.url?scp=84884487128&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84884487128&partnerID=8YFLogxK

U2 - 10.1091/mbc.E13-07-0381

DO - 10.1091/mbc.E13-07-0381

M3 - Article

C2 - 23904270

AN - SCOPUS:84884487128

VL - 24

SP - 2918

EP - 2931

JO - Molecular Biology of the Cell

JF - Molecular Biology of the Cell

SN - 1059-1524

IS - 18

ER -