ERG protein expression in human tumors detected with a rabbit monoclonal antibody

Oksana Yaskiv, Brian P. Rubin, Huiying He, Sara Falzarano, Cristina Magi-Galluzzi, Ming Zhou

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29 Scopus citations

Abstract

Avian v-ets erythroblastosis virus E26 oncogene homolog (ERG) is highly sensitive and specific for endothelial neoplasms and specific for prostate carcinoma. We characterized a rabbit anti-ERG antibody as an immunohistochemical agent to detect ERG expression in various tumors using tissue microarrays with a wide array of epithelial and mesenchymal tumors. ERG was positive in 63 (38%) of 168 prostate carcinomas and negative in all other epithelial tumors. ERG was positive in all 125 vascular lesions. It was also positive in the sarcomatoid component of a high-grade urothelial carcinoma and 6 (40%) of 15 meningiomas. Twelve (80%) of 15 meningiomas were positive for Fli1, including all 6 ERG-positive cases. Positive immunostaining with this antibody is therefore highly specific for prostate carcinoma and vascular lesions, with a few caveats. ERG is rarely detected in nonvascular mesenchymal tumors with this antibody. Furthermore, about 40% of meningiomas are also positive for ERG immunohistochemically, probably because of cross-reactivity with Fli1.

Original languageEnglish (US)
Pages (from-to)803-810
Number of pages8
JournalAmerican journal of clinical pathology
Volume138
Issue number6
DOIs
StatePublished - Dec 1 2012

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Keywords

  • ERG
  • Immunohistochemistry
  • Meningioma
  • Prostate carcinoma
  • Vascular lesion

ASJC Scopus subject areas

  • Pathology and Forensic Medicine

Cite this

Yaskiv, O., Rubin, B. P., He, H., Falzarano, S., Magi-Galluzzi, C., & Zhou, M. (2012). ERG protein expression in human tumors detected with a rabbit monoclonal antibody. American journal of clinical pathology, 138(6), 803-810. https://doi.org/10.1309/AJCP3K5VUFALZTKC