TY - JOUR
T1 - ERK regulates Golgi and centrosome orientation towards the leading edge through GRASP65
AU - Bisel, Blaine
AU - Wang, Yanzhuang
AU - Wei, Jen Hsuan
AU - Xiang, Yi
AU - Tang, Danming
AU - Miron-Mendoza, Miguel
AU - Yoshimura, Shin Ichiro
AU - Nakamura, Nobuhiro
AU - Seemann, Joachim
PY - 2008/9/8
Y1 - 2008/9/8
N2 - Directed cell migration requires the orientation of the Golgi and centrosome toward the leading edge. We show that stimulation of interphase cells with the mitogens epidermal growth factor or lysophosphatidic acid activates the extracellular signal-regulated kinase (ERK), which phosphorylates the Golgi structural protein GRASP65 at serine 277. Expression of a GRASP65 Ser277 to alanine mutant or a GRASP65 1-201 truncation mutant, neither of which can be phosphorylated by ERK, prevents Golgi orientation to the leading edge in a wound assay. We show that phosphorylation of GRASP65 with recombinant ERK leads to the loss of GRASP65 oligomerization and causes Golgi cisternal unstacking. Furthermore, preventing Golgi polarization by expressing mutated GRASP65 inhibits centrosome orientation, which is rescued upon disassembly of the Golgi structure by brefeldin A. We conclude that Golgi remodeling, mediated by phosphoryl ation of GRASP65 by ERK, is critical for the establishment of cell polarity in migrating cells.
AB - Directed cell migration requires the orientation of the Golgi and centrosome toward the leading edge. We show that stimulation of interphase cells with the mitogens epidermal growth factor or lysophosphatidic acid activates the extracellular signal-regulated kinase (ERK), which phosphorylates the Golgi structural protein GRASP65 at serine 277. Expression of a GRASP65 Ser277 to alanine mutant or a GRASP65 1-201 truncation mutant, neither of which can be phosphorylated by ERK, prevents Golgi orientation to the leading edge in a wound assay. We show that phosphorylation of GRASP65 with recombinant ERK leads to the loss of GRASP65 oligomerization and causes Golgi cisternal unstacking. Furthermore, preventing Golgi polarization by expressing mutated GRASP65 inhibits centrosome orientation, which is rescued upon disassembly of the Golgi structure by brefeldin A. We conclude that Golgi remodeling, mediated by phosphoryl ation of GRASP65 by ERK, is critical for the establishment of cell polarity in migrating cells.
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U2 - 10.1083/jcb.200805045
DO - 10.1083/jcb.200805045
M3 - Article
C2 - 18762583
AN - SCOPUS:51649100852
SN - 0021-9525
VL - 182
SP - 837
EP - 843
JO - Journal of Cell Biology
JF - Journal of Cell Biology
IS - 5
ER -