Abstract
We have previously demonstrated an involvement of MEK5 and ERK5 in RafBXB-stimulated focus formation in NIH3T3 cells. We find here that MEK5 and ERK5 cooperate with the RafBXB effectors MEK1/2 and ERK1/2 to induce foci. To further understand MEK5-ERK5-dependent signaling, we examined potential MEK5-ERK5 effectors that might influence focus-forming activity. Consistent with results from our focus-formation assays, constitutively active variants of MEK5 and MEK1 synergize to activate NF-κB, and MEK5 and ERK5 are required for activation of NF-κB by RafBXB. The MEK5-ERK5 pathway is also sufficient to activate both NF-κB and p90 ribosomal S6 kinase. Our results support the hypothesis that NF-κB and p90 ribosomal S6 kinase are involved in MEK5-ERK5-dependent focus formation and may serve as integration points for ERK5 and ERK1/2 signaling.
Original language | English (US) |
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Pages (from-to) | 7927-7931 |
Number of pages | 5 |
Journal | Journal of Biological Chemistry |
Volume | 276 |
Issue number | 11 |
DOIs | |
State | Published - Mar 16 2001 |
ASJC Scopus subject areas
- Biochemistry
- Molecular Biology
- Cell Biology