Erratum: DAPLE protein inhibits nucleotide exchange on Gas and Gaq via the same motif that activates Ga_i(J. Biol. Chem. (2020) 295 (2270-2284) DOI: 10.1074/jbc.RA119.011648)

MgCl₂ was omitted as a reagent in two buffers described under “In vitro protein-binding assays with GST-fused proteins” section (under “Experimental procedures”). “Beads were washed twice with PBS and resuspended in 300 ml of binding buffer (50 mM Tris-HCl (pH 7.4), 100 mM NaCl, 0.4% (vol:vol) NP-40, 5 mM EDTA, 2 mM DTT)” should read as “Beads were washed twice with PBS and resuspended in 300 ml of binding buffer (50 mM Tris-HCl (pH 7.4), 100 mM NaCl, 10 mM MgCl₂, 0.4% (vol:vol) NP-40, 5 mM EDTA, 2 mM DTT).” “Beads were washed four times with 1 ml of wash buffer (4.3 mM Na₂HPO₄, 1.4 mM KH₂PO₄, pH 7.4, 137 mM NaCl, 2.7 mM KCl, 0.1% (vol:vol) Tween-20, 5 mM EDTA, 1 mM DTT)” should read as “Beads were washed four times with 1 ml of wash buffer (4.3 mM Na₂HPO₄, 1.4 mM KH₂PO₄, pH 7.4, 137 mM NaCl, 2.7 mM KCl, 0.1% (vol:vol) Tween-20, 10 mM MgCl₂, 5 mM EDTA, 1 mM DTT)”.