Erratum: DAPLE protein inhibits nucleotide exchange on Gas and Gaq via the same motif that activates Gai(J. Biol. Chem. (2020) 295 (2270-2284) DOI: 10.1074/jbc.RA119.011648)

Arthur Marivin, Marcin Maziarz, Jingyi Zhao, Vincent DiGiacomo, Isabel Olmos Calvo, Emily A. Mann, Jason Ear, Juan B. Blanco-Canosa, Elliott M. Ross, Pradipta Ghosh, Mikel Garcia-Marcos

Research output: Contribution to journalComment/debatepeer-review

Abstract

MgCl2 was omitted as a reagent in two buffers described under “In vitro protein-binding assays with GST-fused proteins” section (under “Experimental procedures”). “Beads were washed twice with PBS and resuspended in 300 ml of binding buffer (50 mM Tris-HCl (pH 7.4), 100 mM NaCl, 0.4% (vol:vol) NP-40, 5 mM EDTA, 2 mM DTT)” should read as “Beads were washed twice with PBS and resuspended in 300 ml of binding buffer (50 mM Tris-HCl (pH 7.4), 100 mM NaCl, 10 mM MgCl2, 0.4% (vol:vol) NP-40, 5 mM EDTA, 2 mM DTT).” “Beads were washed four times with 1 ml of wash buffer (4.3 mM Na2HPO4, 1.4 mM KH2PO4, pH 7.4, 137 mM NaCl, 2.7 mM KCl, 0.1% (vol:vol) Tween-20, 5 mM EDTA, 1 mM DTT)” should read as “Beads were washed four times with 1 ml of wash buffer (4.3 mM Na2HPO4, 1.4 mM KH2PO4, pH 7.4, 137 mM NaCl, 2.7 mM KCl, 0.1% (vol:vol) Tween-20, 10 mM MgCl2, 5 mM EDTA, 1 mM DTT)”.

Original languageEnglish (US)
Pages (from-to)8867
Number of pages1
JournalJournal of Biological Chemistry
Volume295
Issue number26
DOIs
StatePublished - Jun 26 2020

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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