Erratum

Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice (The Journal of Biological Chemistry (2009) 284 (11309-11317) DOI: 10.1074/jbc.A116.802400)

Stavros Garantziotis, Zhuowei Li, Erin N. Potts, Koji Kimata, Lisheng Zhuo, Daniel L. Morgan, Rashmin C. Savani, Paul W. Noble, W. Michael Foster, David A. Schwartz, John W. Hollingsworth

Research output: Contribution to journalComment/debate

5 Citations (Scopus)

Abstract

In this article, we provided immunological, histological, and physiological evidence that hyaluronan mediates the development of inflammation and airway hyperresponsiveness (AHR) after ozone inhalation (1). After publication of our article, we became aware that the airway physiology measurements reported in Figs. 5, 6, and 9 of the original manuscript, which were provided by the animal pulmonary physiology laboratory at Duke University, may have been unreliable. We therefore had two separate laboratories, one at Duke University under the supervision of Dr. Herman Staats, and the other at the National Institute of Environmental Health Sciences under the supervision of Dr. Stavros Garantziotis, repeat the experiments in question. These laboratories have replicated the key observation that hyaluronan mediates ozone-induced airway hyperresponsiveness. We again demonstrated that CD44 and inter-α-inhibitor (IαI) are necessary for the development of ozone-induced AHR (Figs. 1 and 2, n = 5-10 per group; p < 0.05). Furthermore, we confirmed that hyaluronan-binding protein (HABP) decreases AHR after ozone exposure (Fig. 3, n = 4-5 per group; p < 0.05). We showed that short fragment hyaluronan (sHA; molecular mass of 100-400 kDa) but not vehicle or high molecular weight hyaluronan (HMW-HA; molecular mass > 1,000 kDa) induces AHR in naïve C57BL/6 mice (Fig. 4, n = 4-6 mice per group; p < 0.05), but not in CD44-deficient mice (Fig. 5, n = 4-8 mice per group; p < 0.05). We were unable to confirm that HMW-HA ameliorates ozone-induced AHR, although there was a trend to that direction (Fig. 6, n = 8-10 mice per group; p = 0.04 but not significant by Holm-Sidak analysis and α = 5%). In conclusion, we report that the repeated work largely validates the originally reported findings. Of note, the major conclusion of this article, i.e., that sHA mediates AHR after oxidative lung injury, has been independently validated in studies performed since this publication. For example, sHA mediates AHR after chlorine gas exposure, whereas HMW-HA or IαI blockade inhibits chlorine-induced AHR (2). Furthermore, exposure to ozone has been independently shown to result in increased levels of hyaluronan in the bronchoalveolar lavage fluid of mice (3) and humans (4, 5). Exposure to ozone was independently shown to fragment hyaluronan from HMW-HA to sHA (6). Furthermore, studies of murine asthma show that TSG-6, which links hyaluronan to IαI, is necessary for the development of inflammatory AHR (7). Finally, CD44 is necessary for the development of sHA-induced AHR in a tracheal ring model (2). Together, these studies support that several models of pulmonary inflammation result in increased levels of sHA, which mediates AHR. In aggregate, our repeated studies confirm the original conclusions of the manuscript, and independent research also supports our originally reported conclusions. We therefore believe that our original report is accurate and valid.

Original languageEnglish (US)
Pages (from-to)19257-19258
Number of pages2
JournalJournal of Biological Chemistry
Volume291
Issue number37
DOIs
StatePublished - Sep 9 2016

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Ozone
Hyaluronic Acid
Ficus
Manuscripts
Chlorine
National Institute of Environmental Health Sciences (U.S.)
Physiology
Bronchoalveolar Lavage Fluid
Lung Injury
Inbred C57BL Mouse
Inhalation
Publications
Pneumonia
Asthma
Gases
Observation
Inflammation
Lung
Research
Animals

ASJC Scopus subject areas

  • Biochemistry
  • Medicine(all)
  • Molecular Biology
  • Cell Biology

Cite this

Erratum : Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice (The Journal of Biological Chemistry (2009) 284 (11309-11317) DOI: 10.1074/jbc.A116.802400). / Garantziotis, Stavros; Li, Zhuowei; Potts, Erin N.; Kimata, Koji; Zhuo, Lisheng; Morgan, Daniel L.; Savani, Rashmin C.; Noble, Paul W.; Foster, W. Michael; Schwartz, David A.; Hollingsworth, John W.

In: Journal of Biological Chemistry, Vol. 291, No. 37, 09.09.2016, p. 19257-19258.

Research output: Contribution to journalComment/debate

Garantziotis, S, Li, Z, Potts, EN, Kimata, K, Zhuo, L, Morgan, DL, Savani, RC, Noble, PW, Foster, WM, Schwartz, DA & Hollingsworth, JW 2016, 'Erratum: Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice (The Journal of Biological Chemistry (2009) 284 (11309-11317) DOI: 10.1074/jbc.A116.802400)', Journal of Biological Chemistry, vol. 291, no. 37, pp. 19257-19258. https://doi.org/10.1074/jbc.A116.802400
Garantziotis, Stavros ; Li, Zhuowei ; Potts, Erin N. ; Kimata, Koji ; Zhuo, Lisheng ; Morgan, Daniel L. ; Savani, Rashmin C. ; Noble, Paul W. ; Foster, W. Michael ; Schwartz, David A. ; Hollingsworth, John W. / Erratum : Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice (The Journal of Biological Chemistry (2009) 284 (11309-11317) DOI: 10.1074/jbc.A116.802400). In: Journal of Biological Chemistry. 2016 ; Vol. 291, No. 37. pp. 19257-19258.
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abstract = "In this article, we provided immunological, histological, and physiological evidence that hyaluronan mediates the development of inflammation and airway hyperresponsiveness (AHR) after ozone inhalation (1). After publication of our article, we became aware that the airway physiology measurements reported in Figs. 5, 6, and 9 of the original manuscript, which were provided by the animal pulmonary physiology laboratory at Duke University, may have been unreliable. We therefore had two separate laboratories, one at Duke University under the supervision of Dr. Herman Staats, and the other at the National Institute of Environmental Health Sciences under the supervision of Dr. Stavros Garantziotis, repeat the experiments in question. These laboratories have replicated the key observation that hyaluronan mediates ozone-induced airway hyperresponsiveness. We again demonstrated that CD44 and inter-α-inhibitor (IαI) are necessary for the development of ozone-induced AHR (Figs. 1 and 2, n = 5-10 per group; p < 0.05). Furthermore, we confirmed that hyaluronan-binding protein (HABP) decreases AHR after ozone exposure (Fig. 3, n = 4-5 per group; p < 0.05). We showed that short fragment hyaluronan (sHA; molecular mass of 100-400 kDa) but not vehicle or high molecular weight hyaluronan (HMW-HA; molecular mass > 1,000 kDa) induces AHR in na{\"i}ve C57BL/6 mice (Fig. 4, n = 4-6 mice per group; p < 0.05), but not in CD44-deficient mice (Fig. 5, n = 4-8 mice per group; p < 0.05). We were unable to confirm that HMW-HA ameliorates ozone-induced AHR, although there was a trend to that direction (Fig. 6, n = 8-10 mice per group; p = 0.04 but not significant by Holm-Sidak analysis and α = 5{\%}). In conclusion, we report that the repeated work largely validates the originally reported findings. Of note, the major conclusion of this article, i.e., that sHA mediates AHR after oxidative lung injury, has been independently validated in studies performed since this publication. For example, sHA mediates AHR after chlorine gas exposure, whereas HMW-HA or IαI blockade inhibits chlorine-induced AHR (2). Furthermore, exposure to ozone has been independently shown to result in increased levels of hyaluronan in the bronchoalveolar lavage fluid of mice (3) and humans (4, 5). Exposure to ozone was independently shown to fragment hyaluronan from HMW-HA to sHA (6). Furthermore, studies of murine asthma show that TSG-6, which links hyaluronan to IαI, is necessary for the development of inflammatory AHR (7). Finally, CD44 is necessary for the development of sHA-induced AHR in a tracheal ring model (2). Together, these studies support that several models of pulmonary inflammation result in increased levels of sHA, which mediates AHR. In aggregate, our repeated studies confirm the original conclusions of the manuscript, and independent research also supports our originally reported conclusions. We therefore believe that our original report is accurate and valid.",
author = "Stavros Garantziotis and Zhuowei Li and Potts, {Erin N.} and Koji Kimata and Lisheng Zhuo and Morgan, {Daniel L.} and Savani, {Rashmin C.} and Noble, {Paul W.} and Foster, {W. Michael} and Schwartz, {David A.} and Hollingsworth, {John W.}",
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TY - JOUR

T1 - Erratum

T2 - Hyaluronan mediates ozone-induced airway hyperresponsiveness in mice (The Journal of Biological Chemistry (2009) 284 (11309-11317) DOI: 10.1074/jbc.A116.802400)

AU - Garantziotis, Stavros

AU - Li, Zhuowei

AU - Potts, Erin N.

AU - Kimata, Koji

AU - Zhuo, Lisheng

AU - Morgan, Daniel L.

AU - Savani, Rashmin C.

AU - Noble, Paul W.

AU - Foster, W. Michael

AU - Schwartz, David A.

AU - Hollingsworth, John W.

PY - 2016/9/9

Y1 - 2016/9/9

N2 - In this article, we provided immunological, histological, and physiological evidence that hyaluronan mediates the development of inflammation and airway hyperresponsiveness (AHR) after ozone inhalation (1). After publication of our article, we became aware that the airway physiology measurements reported in Figs. 5, 6, and 9 of the original manuscript, which were provided by the animal pulmonary physiology laboratory at Duke University, may have been unreliable. We therefore had two separate laboratories, one at Duke University under the supervision of Dr. Herman Staats, and the other at the National Institute of Environmental Health Sciences under the supervision of Dr. Stavros Garantziotis, repeat the experiments in question. These laboratories have replicated the key observation that hyaluronan mediates ozone-induced airway hyperresponsiveness. We again demonstrated that CD44 and inter-α-inhibitor (IαI) are necessary for the development of ozone-induced AHR (Figs. 1 and 2, n = 5-10 per group; p < 0.05). Furthermore, we confirmed that hyaluronan-binding protein (HABP) decreases AHR after ozone exposure (Fig. 3, n = 4-5 per group; p < 0.05). We showed that short fragment hyaluronan (sHA; molecular mass of 100-400 kDa) but not vehicle or high molecular weight hyaluronan (HMW-HA; molecular mass > 1,000 kDa) induces AHR in naïve C57BL/6 mice (Fig. 4, n = 4-6 mice per group; p < 0.05), but not in CD44-deficient mice (Fig. 5, n = 4-8 mice per group; p < 0.05). We were unable to confirm that HMW-HA ameliorates ozone-induced AHR, although there was a trend to that direction (Fig. 6, n = 8-10 mice per group; p = 0.04 but not significant by Holm-Sidak analysis and α = 5%). In conclusion, we report that the repeated work largely validates the originally reported findings. Of note, the major conclusion of this article, i.e., that sHA mediates AHR after oxidative lung injury, has been independently validated in studies performed since this publication. For example, sHA mediates AHR after chlorine gas exposure, whereas HMW-HA or IαI blockade inhibits chlorine-induced AHR (2). Furthermore, exposure to ozone has been independently shown to result in increased levels of hyaluronan in the bronchoalveolar lavage fluid of mice (3) and humans (4, 5). Exposure to ozone was independently shown to fragment hyaluronan from HMW-HA to sHA (6). Furthermore, studies of murine asthma show that TSG-6, which links hyaluronan to IαI, is necessary for the development of inflammatory AHR (7). Finally, CD44 is necessary for the development of sHA-induced AHR in a tracheal ring model (2). Together, these studies support that several models of pulmonary inflammation result in increased levels of sHA, which mediates AHR. In aggregate, our repeated studies confirm the original conclusions of the manuscript, and independent research also supports our originally reported conclusions. We therefore believe that our original report is accurate and valid.

AB - In this article, we provided immunological, histological, and physiological evidence that hyaluronan mediates the development of inflammation and airway hyperresponsiveness (AHR) after ozone inhalation (1). After publication of our article, we became aware that the airway physiology measurements reported in Figs. 5, 6, and 9 of the original manuscript, which were provided by the animal pulmonary physiology laboratory at Duke University, may have been unreliable. We therefore had two separate laboratories, one at Duke University under the supervision of Dr. Herman Staats, and the other at the National Institute of Environmental Health Sciences under the supervision of Dr. Stavros Garantziotis, repeat the experiments in question. These laboratories have replicated the key observation that hyaluronan mediates ozone-induced airway hyperresponsiveness. We again demonstrated that CD44 and inter-α-inhibitor (IαI) are necessary for the development of ozone-induced AHR (Figs. 1 and 2, n = 5-10 per group; p < 0.05). Furthermore, we confirmed that hyaluronan-binding protein (HABP) decreases AHR after ozone exposure (Fig. 3, n = 4-5 per group; p < 0.05). We showed that short fragment hyaluronan (sHA; molecular mass of 100-400 kDa) but not vehicle or high molecular weight hyaluronan (HMW-HA; molecular mass > 1,000 kDa) induces AHR in naïve C57BL/6 mice (Fig. 4, n = 4-6 mice per group; p < 0.05), but not in CD44-deficient mice (Fig. 5, n = 4-8 mice per group; p < 0.05). We were unable to confirm that HMW-HA ameliorates ozone-induced AHR, although there was a trend to that direction (Fig. 6, n = 8-10 mice per group; p = 0.04 but not significant by Holm-Sidak analysis and α = 5%). In conclusion, we report that the repeated work largely validates the originally reported findings. Of note, the major conclusion of this article, i.e., that sHA mediates AHR after oxidative lung injury, has been independently validated in studies performed since this publication. For example, sHA mediates AHR after chlorine gas exposure, whereas HMW-HA or IαI blockade inhibits chlorine-induced AHR (2). Furthermore, exposure to ozone has been independently shown to result in increased levels of hyaluronan in the bronchoalveolar lavage fluid of mice (3) and humans (4, 5). Exposure to ozone was independently shown to fragment hyaluronan from HMW-HA to sHA (6). Furthermore, studies of murine asthma show that TSG-6, which links hyaluronan to IαI, is necessary for the development of inflammatory AHR (7). Finally, CD44 is necessary for the development of sHA-induced AHR in a tracheal ring model (2). Together, these studies support that several models of pulmonary inflammation result in increased levels of sHA, which mediates AHR. In aggregate, our repeated studies confirm the original conclusions of the manuscript, and independent research also supports our originally reported conclusions. We therefore believe that our original report is accurate and valid.

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