Erythropoietin improves synaptic transmission during and following ischemia in rat hippocampal slice cultures

Astrid Weber; Rolf F. Maier; Ulrike Hoffmann; Martin Grips; Marc Hoppenz; Ayse G. Aktas; Uwe Heinemann; Michael Obladen; Sebastian Schuchmann

doi:10.1016/S0006-8993(02)03604-1

Erythropoietin improves synaptic transmission during and following ischemia in rat hippocampal slice cultures

Astrid Weber, Rolf F. Maier, Ulrike Hoffmann, Martin Grips, Marc Hoppenz, Ayse G. Aktas, Uwe Heinemann, Michael Obladen, Sebastian Schuchmann

Research output: Contribution to journal › Article › peer-review

51 Scopus citations

Abstract

Erythropoietin (EPO) prevents neuronal damage following ischemic, metabolic, and excitotoxic stress. In this study evoked extracellular field potentials (FP) were used to investigate the effect of EPO on synaptic transmission in hippocampal slice cultures. EPO treated cultured slices (40 units/ml for 48 h) showed significantly increased FP during and following oxygen and glucose deprivation compared with untreated control slices. The addition of the Jak2 inhibitor AG490 (50 μM for 48 h) blocked the EPO effect. These data suggest that EPO improves synaptic transmission during and following ischemia in hippocampal slice cultures.

Original language	English (US)
Pages (from-to)	305-311
Number of pages	7
Journal	Brain Research
Volume	958
Issue number	2
DOIs	https://doi.org/10.1016/S0006-8993(02)03604-1
State	Published - Dec 27 2002
Externally published	Yes

Keywords

Erythropoietin
Hippocampal slice culture
Ischemia
Neuroprotection
Synaptic transmission

ASJC Scopus subject areas

Neuroscience(all)
Molecular Biology
Clinical Neurology
Developmental Biology

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10.1016/S0006-8993(02)03604-1

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title = "Erythropoietin improves synaptic transmission during and following ischemia in rat hippocampal slice cultures",

abstract = "Erythropoietin (EPO) prevents neuronal damage following ischemic, metabolic, and excitotoxic stress. In this study evoked extracellular field potentials (FP) were used to investigate the effect of EPO on synaptic transmission in hippocampal slice cultures. EPO treated cultured slices (40 units/ml for 48 h) showed significantly increased FP during and following oxygen and glucose deprivation compared with untreated control slices. The addition of the Jak2 inhibitor AG490 (50 μM for 48 h) blocked the EPO effect. These data suggest that EPO improves synaptic transmission during and following ischemia in hippocampal slice cultures.",

keywords = "Erythropoietin, Hippocampal slice culture, Ischemia, Neuroprotection, Synaptic transmission",

author = "Astrid Weber and Maier, {Rolf F.} and Ulrike Hoffmann and Martin Grips and Marc Hoppenz and Aktas, {Ayse G.} and Uwe Heinemann and Michael Obladen and Sebastian Schuchmann",

note = "Funding Information: We wish to thank J. Graulich for his crucial initiation to the collaboration group. We gratefully acknowledge technical support from S. Gabriel, H.J. Gabriel, S. Latta, and H. Siegmund and help with the preparation of the manuscript from S. Sch{\"u}rmann. This study was supported by a grant of the Wilhelm Sander-Stiftung, M{\"u}nchen (No. 2000.091.1) and a grant of the Sonnenfeld-Stiftung, Berlin.",

year = "2002",

month = dec,

day = "27",

doi = "10.1016/S0006-8993(02)03604-1",

language = "English (US)",

volume = "958",

pages = "305--311",

journal = "Brain Research",

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number = "2",

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TY - JOUR

T1 - Erythropoietin improves synaptic transmission during and following ischemia in rat hippocampal slice cultures

AU - Weber, Astrid

AU - Maier, Rolf F.

AU - Hoffmann, Ulrike

AU - Grips, Martin

AU - Hoppenz, Marc

AU - Aktas, Ayse G.

AU - Heinemann, Uwe

AU - Obladen, Michael

AU - Schuchmann, Sebastian

N1 - Funding Information: We wish to thank J. Graulich for his crucial initiation to the collaboration group. We gratefully acknowledge technical support from S. Gabriel, H.J. Gabriel, S. Latta, and H. Siegmund and help with the preparation of the manuscript from S. Schürmann. This study was supported by a grant of the Wilhelm Sander-Stiftung, München (No. 2000.091.1) and a grant of the Sonnenfeld-Stiftung, Berlin.

PY - 2002/12/27

Y1 - 2002/12/27

N2 - Erythropoietin (EPO) prevents neuronal damage following ischemic, metabolic, and excitotoxic stress. In this study evoked extracellular field potentials (FP) were used to investigate the effect of EPO on synaptic transmission in hippocampal slice cultures. EPO treated cultured slices (40 units/ml for 48 h) showed significantly increased FP during and following oxygen and glucose deprivation compared with untreated control slices. The addition of the Jak2 inhibitor AG490 (50 μM for 48 h) blocked the EPO effect. These data suggest that EPO improves synaptic transmission during and following ischemia in hippocampal slice cultures.

AB - Erythropoietin (EPO) prevents neuronal damage following ischemic, metabolic, and excitotoxic stress. In this study evoked extracellular field potentials (FP) were used to investigate the effect of EPO on synaptic transmission in hippocampal slice cultures. EPO treated cultured slices (40 units/ml for 48 h) showed significantly increased FP during and following oxygen and glucose deprivation compared with untreated control slices. The addition of the Jak2 inhibitor AG490 (50 μM for 48 h) blocked the EPO effect. These data suggest that EPO improves synaptic transmission during and following ischemia in hippocampal slice cultures.

KW - Erythropoietin

KW - Hippocampal slice culture

KW - Ischemia

KW - Neuroprotection

KW - Synaptic transmission

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AN - SCOPUS:0037184707

SN - 0006-8993

VL - 958

SP - 305

EP - 311

JO - Brain Research

JF - Brain Research

IS - 2

ER -

Erythropoietin improves synaptic transmission during and following ischemia in rat hippocampal slice cultures

Abstract

Keywords

ASJC Scopus subject areas

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