Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites

Yamamoto Denise, T. Hernandes Rodrigo, Maria A Liberatore Ana, Cecilia M. Abe, B. Souza De Rodrigo, T. Romão Fabiano, Sperandio Vanessa, H. Koh Ivan, A. T Gomes Tânia

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

Diarrhea is the second leading cause of death of children up to five years old in the developing countries. Among the etiological diarrheal agents are atypical enteropathogenic Escherichia coli (aEPEC), one of the diarrheagenic E. coli pathotypes that affects children and adults, even in developed countries. Currently, genotypic and biochemical approaches have helped to demonstrate that some strains classified as aEPEC are actually E. albertii, a recently recognized human enteropathogen. Studies on particular strains are necessary to explore their virulence potential in order to further understand the underlying mechanisms of E. albertii infections. Here we demonstrated for the first time that infection of fragments of rat intestinal mucosa is a useful tool to study the initial steps of E. albertii colonization. We also observed that an E. albertii strain can translocate from the intestinal lumen to Mesenteric Lymph Nodes and liver in a rat model. Based on our finding of bacterial translocation, we investigated how E. albertii might cross the intestinal epithelium by performing infections of M-like cells in vitro to identify the potential in vivo translocation route. Altogether, our approaches allowed us to draft a general E. albertii infection route from the colonization till the bacterial spreading in vivo.

Original languageEnglish (US)
Article numbere0171385
JournalPLoS One
Volume12
Issue number2
DOIs
StatePublished - Feb 1 2017

Fingerprint

Escherichia albertii
enteropathogens
Escherichia
Enterocytes
enterocytes
Escherichia coli
Rats
Enteropathogenic Escherichia coli
rats
Intestinal Mucosa
Infection
enteropathogenic Escherichia coli
intestinal mucosa
Bacterial Translocation
Developing countries
infection
Liver
Cells
Developed Countries
Developing Countries

ASJC Scopus subject areas

  • Medicine(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Agricultural and Biological Sciences(all)

Cite this

Denise, Y., Rodrigo, T. H., Ana, M. A. L., Abe, C. M., De Rodrigo, B. S., Fabiano, T. R., ... Tânia, A. T. G. (2017). Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites. PLoS One, 12(2), [e0171385]. https://doi.org/10.1371/journal.pone.0171385

Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites. / Denise, Yamamoto; Rodrigo, T. Hernandes; Ana, Maria A Liberatore; Abe, Cecilia M.; De Rodrigo, B. Souza; Fabiano, T. Romão; Vanessa, Sperandio; Ivan, H. Koh; Tânia, A. T Gomes.

In: PLoS One, Vol. 12, No. 2, e0171385, 01.02.2017.

Research output: Contribution to journalArticle

Denise, Y, Rodrigo, TH, Ana, MAL, Abe, CM, De Rodrigo, BS, Fabiano, TR, Vanessa, S, Ivan, HK & Tânia, ATG 2017, 'Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites', PLoS One, vol. 12, no. 2, e0171385. https://doi.org/10.1371/journal.pone.0171385
Denise, Yamamoto ; Rodrigo, T. Hernandes ; Ana, Maria A Liberatore ; Abe, Cecilia M. ; De Rodrigo, B. Souza ; Fabiano, T. Romão ; Vanessa, Sperandio ; Ivan, H. Koh ; Tânia, A. T Gomes. / Escherichia albertii, a novel human enteropathogen, colonizes rat enterocytes and translocates to extra-intestinal sites. In: PLoS One. 2017 ; Vol. 12, No. 2.
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