Essential Role for OspA/B in the Life Cycle of the Lyme Disease Spirochete

Xiaofeng F. Yang, Utpal Pal, Sophie M. Alani, Erol Fikrig, Michael V. Norgard

Research output: Contribution to journalArticle

224 Citations (Scopus)

Abstract

The molecular basis of how Borrelia burgdorferi (Bb), the Lyme disease spirochete, maintains itself in nature via a complex life cycle in ticks and mammals is poorly understood. Outer surface (lipo)protein A (OspA) of Bb has been the most intensively studied of all borrelial molecular constituents, and hence, much has been speculated about the potential role(s) of OspA in the life cycle of Bb. However, the precise function of OspA (along with that of its close relative and operonic partner, outer surface [lipo]protein B [OspB]) heretofore has not been directly determined, due primarily to the inability to generate an OspA/B-deficient mutant from a virulent strain of Bb. In this study, we created an OspA/B-deficient mutant of an infectious human isolate of Bb (strain 297) and found that OspA/B function was not required for either Bb infection of mice or accompanying tissue pathology. However, OspA/B function was essential for Bb colonization of and survival within tick midguts, events crucial for sustaining Bb in its natural enzootic life cycle.

Original languageEnglish (US)
Pages (from-to)641-648
Number of pages8
JournalJournal of Experimental Medicine
Volume199
Issue number5
DOIs
StatePublished - Mar 1 2004

Fingerprint

Borrelia burgdorferi
Life Cycle Stages
Ticks
Membrane Proteins
Borrelia Infections
Staphylococcal Protein A
Mammals
Pathology
Survival

Keywords

  • Arthropod vector
  • Borrelia burgdorferi
  • Lipoprotein
  • Lyme disease
  • OspA

ASJC Scopus subject areas

  • Immunology

Cite this

Essential Role for OspA/B in the Life Cycle of the Lyme Disease Spirochete. / Yang, Xiaofeng F.; Pal, Utpal; Alani, Sophie M.; Fikrig, Erol; Norgard, Michael V.

In: Journal of Experimental Medicine, Vol. 199, No. 5, 01.03.2004, p. 641-648.

Research output: Contribution to journalArticle

Yang, Xiaofeng F. ; Pal, Utpal ; Alani, Sophie M. ; Fikrig, Erol ; Norgard, Michael V. / Essential Role for OspA/B in the Life Cycle of the Lyme Disease Spirochete. In: Journal of Experimental Medicine. 2004 ; Vol. 199, No. 5. pp. 641-648.
@article{d9c2b622cf3644329d40b53c113e794f,
title = "Essential Role for OspA/B in the Life Cycle of the Lyme Disease Spirochete",
abstract = "The molecular basis of how Borrelia burgdorferi (Bb), the Lyme disease spirochete, maintains itself in nature via a complex life cycle in ticks and mammals is poorly understood. Outer surface (lipo)protein A (OspA) of Bb has been the most intensively studied of all borrelial molecular constituents, and hence, much has been speculated about the potential role(s) of OspA in the life cycle of Bb. However, the precise function of OspA (along with that of its close relative and operonic partner, outer surface [lipo]protein B [OspB]) heretofore has not been directly determined, due primarily to the inability to generate an OspA/B-deficient mutant from a virulent strain of Bb. In this study, we created an OspA/B-deficient mutant of an infectious human isolate of Bb (strain 297) and found that OspA/B function was not required for either Bb infection of mice or accompanying tissue pathology. However, OspA/B function was essential for Bb colonization of and survival within tick midguts, events crucial for sustaining Bb in its natural enzootic life cycle.",
keywords = "Arthropod vector, Borrelia burgdorferi, Lipoprotein, Lyme disease, OspA",
author = "Yang, {Xiaofeng F.} and Utpal Pal and Alani, {Sophie M.} and Erol Fikrig and Norgard, {Michael V.}",
year = "2004",
month = "3",
day = "1",
doi = "10.1084/jem.20031960",
language = "English (US)",
volume = "199",
pages = "641--648",
journal = "Journal of Experimental Medicine",
issn = "0022-1007",
publisher = "Rockefeller University Press",
number = "5",

}

TY - JOUR

T1 - Essential Role for OspA/B in the Life Cycle of the Lyme Disease Spirochete

AU - Yang, Xiaofeng F.

AU - Pal, Utpal

AU - Alani, Sophie M.

AU - Fikrig, Erol

AU - Norgard, Michael V.

PY - 2004/3/1

Y1 - 2004/3/1

N2 - The molecular basis of how Borrelia burgdorferi (Bb), the Lyme disease spirochete, maintains itself in nature via a complex life cycle in ticks and mammals is poorly understood. Outer surface (lipo)protein A (OspA) of Bb has been the most intensively studied of all borrelial molecular constituents, and hence, much has been speculated about the potential role(s) of OspA in the life cycle of Bb. However, the precise function of OspA (along with that of its close relative and operonic partner, outer surface [lipo]protein B [OspB]) heretofore has not been directly determined, due primarily to the inability to generate an OspA/B-deficient mutant from a virulent strain of Bb. In this study, we created an OspA/B-deficient mutant of an infectious human isolate of Bb (strain 297) and found that OspA/B function was not required for either Bb infection of mice or accompanying tissue pathology. However, OspA/B function was essential for Bb colonization of and survival within tick midguts, events crucial for sustaining Bb in its natural enzootic life cycle.

AB - The molecular basis of how Borrelia burgdorferi (Bb), the Lyme disease spirochete, maintains itself in nature via a complex life cycle in ticks and mammals is poorly understood. Outer surface (lipo)protein A (OspA) of Bb has been the most intensively studied of all borrelial molecular constituents, and hence, much has been speculated about the potential role(s) of OspA in the life cycle of Bb. However, the precise function of OspA (along with that of its close relative and operonic partner, outer surface [lipo]protein B [OspB]) heretofore has not been directly determined, due primarily to the inability to generate an OspA/B-deficient mutant from a virulent strain of Bb. In this study, we created an OspA/B-deficient mutant of an infectious human isolate of Bb (strain 297) and found that OspA/B function was not required for either Bb infection of mice or accompanying tissue pathology. However, OspA/B function was essential for Bb colonization of and survival within tick midguts, events crucial for sustaining Bb in its natural enzootic life cycle.

KW - Arthropod vector

KW - Borrelia burgdorferi

KW - Lipoprotein

KW - Lyme disease

KW - OspA

UR - http://www.scopus.com/inward/record.url?scp=1542283705&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=1542283705&partnerID=8YFLogxK

U2 - 10.1084/jem.20031960

DO - 10.1084/jem.20031960

M3 - Article

C2 - 14981112

AN - SCOPUS:1542283705

VL - 199

SP - 641

EP - 648

JO - Journal of Experimental Medicine

JF - Journal of Experimental Medicine

SN - 0022-1007

IS - 5

ER -