Essential role for protein kinase D family kinases in the regulation of class II histone deacetylases in B lymphocytes

Sharon A. Matthews, Ping Liu, Martin Spitaler, Eric N. Olson, Timothy A. McKinsey, Doreen A. Cantrell, Andrew M. Scharenberg

Research output: Contribution to journalArticle

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Abstract

We have taken a knockout approach to interrogate the function of protein kinase D (PKD) serine/threonine kinases in lymphocytes. DT40 B cells express two PKD family members, PKD1 and PKD3, which are both rapidly activated by the B-cell antigen receptor (BCR). DT40 cells with single or dual deletions of PKD1 and/or PKD3 were viable, allowing the role of individual PKD isoforms in BCR signal transduction to be assessed. One proposed downstream target for PKD1 in lymphocytes is the class II histone deacetylases (HDACs). Regulation of chromatin accessibility via class II histone deacetylases is an important mechanism controlling gene expression patterns, but the molecules that control this key process in B cells are not known. Herein, we show that phosphorylation and nuclear export of the class II histone deacetylases HDAC5 and HDAC7 are rapidly induced following ligation of the BCR or after treatment with phorbol esters (a diacylglycerol mimetic). Loss of either PKD1 or PKD3 had no impact on HDAC phosphorylation, but loss of both PKD1 and PKD3 abrogated antigen receptor-induced class II HDAC5/7 phosphorylation and nuclear export. These studies reveal an essential and redundant role for PKD enzymes in controlling class II HDACs in B lymphocytes and suggest that PKD serine kinases are a critical link between the BCR and epigenetic control of chromatin.

Original languageEnglish (US)
Pages (from-to)1569-1577
Number of pages9
JournalMolecular and Cellular Biology
Volume26
Issue number4
DOIs
StatePublished - Feb 2006

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Histone Deacetylases
B-Cell Antigen Receptors
B-Lymphocytes
Phosphotransferases
Cell Nucleus Active Transport
Protein-Serine-Threonine Kinases
Phosphorylation
4 alpha-glucanotransferase
Chromatin
Lymphocytes
Antigen Receptors
Diglycerides
Phorbol Esters
Epigenomics
Ligation
Signal Transduction
Protein Isoforms
protein kinase D
Gene Expression

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Cell Biology

Cite this

Essential role for protein kinase D family kinases in the regulation of class II histone deacetylases in B lymphocytes. / Matthews, Sharon A.; Liu, Ping; Spitaler, Martin; Olson, Eric N.; McKinsey, Timothy A.; Cantrell, Doreen A.; Scharenberg, Andrew M.

In: Molecular and Cellular Biology, Vol. 26, No. 4, 02.2006, p. 1569-1577.

Research output: Contribution to journalArticle

Matthews, Sharon A. ; Liu, Ping ; Spitaler, Martin ; Olson, Eric N. ; McKinsey, Timothy A. ; Cantrell, Doreen A. ; Scharenberg, Andrew M. / Essential role for protein kinase D family kinases in the regulation of class II histone deacetylases in B lymphocytes. In: Molecular and Cellular Biology. 2006 ; Vol. 26, No. 4. pp. 1569-1577.
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