Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals

Chantale Lacelle, Eugenia Wang

Research output: Contribution to journalArticle

3 Scopus citations

Abstract

In population studies involving peripheral blood samples from nonagenarian and centenarian donors, the amount of biological material available for research is restricted, as only a few millilitres of blood can be obtained from extremely old donors without significantly compromising their health. Here we describe a protocol to immortalize small amounts of total frozen blood from extremely old donors (90+) using the Epstein-Barr virus, despite the low level of circulating B cells present in nonagenarian and centenarian blood samples. This methodology provides a unique way to maximize resources of biological material available for research.

Original languageEnglish (US)
Pages (from-to)1415-1418
Number of pages4
JournalMechanisms of Ageing and Development
Volume123
Issue number10
DOIs
StatePublished - Jul 1 2002

Keywords

  • Cell lines
  • EBV
  • Extremely old
  • Longevity

ASJC Scopus subject areas

  • Aging
  • Developmental Biology

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