Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals

Chantale Lacelle; Eugenia Wang

doi:10.1016/S0047-6374(02)00074-X

Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals

Chantale Lacelle, Eugenia Wang

Research output: Contribution to journal › Article › peer-review

3 Scopus citations

Abstract

In population studies involving peripheral blood samples from nonagenarian and centenarian donors, the amount of biological material available for research is restricted, as only a few millilitres of blood can be obtained from extremely old donors without significantly compromising their health. Here we describe a protocol to immortalize small amounts of total frozen blood from extremely old donors (90+) using the Epstein-Barr virus, despite the low level of circulating B cells present in nonagenarian and centenarian blood samples. This methodology provides a unique way to maximize resources of biological material available for research.

Original language	English (US)
Pages (from-to)	1415-1418
Number of pages	4
Journal	Mechanisms of Ageing and Development
Volume	123
Issue number	10
DOIs	https://doi.org/10.1016/S0047-6374(02)00074-X
State	Published - Jul 2002

Keywords

Cell lines
EBV
Extremely old
Longevity

ASJC Scopus subject areas

Aging
Developmental Biology

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10.1016/S0047-6374(02)00074-X

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title = "Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals",

abstract = "In population studies involving peripheral blood samples from nonagenarian and centenarian donors, the amount of biological material available for research is restricted, as only a few millilitres of blood can be obtained from extremely old donors without significantly compromising their health. Here we describe a protocol to immortalize small amounts of total frozen blood from extremely old donors (90+) using the Epstein-Barr virus, despite the low level of circulating B cells present in nonagenarian and centenarian blood samples. This methodology provides a unique way to maximize resources of biological material available for research.",

keywords = "Cell lines, EBV, Extremely old, Longevity",

author = "Chantale Lacelle and Eugenia Wang",

note = "Funding Information: The authors thank Mr. Alan N. Bloch for proofreading this manuscript. This work was supported by National Institutes of Health Grant R37AG07444 from the National Institute on Aging to EW, and by the Defence Advanced Research Project Agency (DARPA) of the Department of Defence of the United States of America (also to EW). ",

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T1 - Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals

AU - Lacelle, Chantale

AU - Wang, Eugenia

N1 - Funding Information: The authors thank Mr. Alan N. Bloch for proofreading this manuscript. This work was supported by National Institutes of Health Grant R37AG07444 from the National Institute on Aging to EW, and by the Defence Advanced Research Project Agency (DARPA) of the Department of Defence of the United States of America (also to EW).

PY - 2002/7

Y1 - 2002/7

N2 - In population studies involving peripheral blood samples from nonagenarian and centenarian donors, the amount of biological material available for research is restricted, as only a few millilitres of blood can be obtained from extremely old donors without significantly compromising their health. Here we describe a protocol to immortalize small amounts of total frozen blood from extremely old donors (90+) using the Epstein-Barr virus, despite the low level of circulating B cells present in nonagenarian and centenarian blood samples. This methodology provides a unique way to maximize resources of biological material available for research.

AB - In population studies involving peripheral blood samples from nonagenarian and centenarian donors, the amount of biological material available for research is restricted, as only a few millilitres of blood can be obtained from extremely old donors without significantly compromising their health. Here we describe a protocol to immortalize small amounts of total frozen blood from extremely old donors (90+) using the Epstein-Barr virus, despite the low level of circulating B cells present in nonagenarian and centenarian blood samples. This methodology provides a unique way to maximize resources of biological material available for research.

KW - Cell lines

KW - EBV

KW - Extremely old

KW - Longevity

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Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals

Abstract

Keywords

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