Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals

Chantale Lacelle, Eugenia Wang

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

In population studies involving peripheral blood samples from nonagenarian and centenarian donors, the amount of biological material available for research is restricted, as only a few millilitres of blood can be obtained from extremely old donors without significantly compromising their health. Here we describe a protocol to immortalize small amounts of total frozen blood from extremely old donors (90+) using the Epstein-Barr virus, despite the low level of circulating B cells present in nonagenarian and centenarian blood samples. This methodology provides a unique way to maximize resources of biological material available for research.

Original languageEnglish (US)
Pages (from-to)1415-1418
Number of pages4
JournalMechanisms of Ageing and Development
Volume123
Issue number10
DOIs
StatePublished - Jul 2002

Fingerprint

Blood
Cells
Cell Line
Tissue Donors
Biological materials
Human Herpesvirus 4
Viruses
Research
B-Lymphocytes
Health
Population

Keywords

  • Cell lines
  • EBV
  • Extremely old
  • Longevity

ASJC Scopus subject areas

  • Aging
  • Biochemistry
  • Developmental Biology
  • Developmental Neuroscience

Cite this

Establishing lymphoblastoid cell lines from frozen blood of extremely old individuals. / Lacelle, Chantale; Wang, Eugenia.

In: Mechanisms of Ageing and Development, Vol. 123, No. 10, 07.2002, p. 1415-1418.

Research output: Contribution to journalArticle

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