Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene

A. F. Gazdar, H. K. Oie, I. R. Kirsch, G. F. Hollis

Research output: Contribution to journalArticle

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Abstract

Using a serum-free defined medium, we have established a human cell line, NCI-H929, from a malignant effusion occurring in a patient with IgAk myeloma. The cultured cells have the morphologic, ultrastructural, biochemical, immunologic, and cytochemical features of plasma cells. The cells have rearranged alpha and kappa genes and synthesize and secrete high amounts of IgAk (>80 μg/106 cells per 24 hours). The cells express surface immunoglobulin (alpha and kappa), the plasma cell antigen PCA-1, the transferrin receptor (T9) and T10 but lack antigens associated with earlier stages of B cell development (HLA-DR, B1, B2, B4, CALLA), as well as other leukocyte-macrophage antigens and Epstein-Barr virus (EBV) nuclear antigen. Although molecular studies confirm that both the tumor and cultured cells are derived from the same clone of malignant B cells, the tumor cells were predominantly near-diploid, whereas the cultured cells are predominantly near-tetraploid with six copies of chromosome 8, four to six of which have an 8q+ abnormality. However, both the tumor and the culturd cells have a rearrangement of the cellular c-myc prot-oncogene (located at 8q24) and express c-myc RNA. Although a modest number of human 'plasmacytoid' cell lines have been established, most are lymphoblastoid lines lacking plasma cell features, while others appear to be early secretory cells. In contrast, NCI-H929 is a differentiated, highly secretory human plasma cell line.

Original languageEnglish (US)
Pages (from-to)1542-1549
Number of pages8
JournalBlood
Volume67
Issue number6
StatePublished - 1986

Fingerprint

Plasma (human)
myc Genes
Proto-Oncogenes
Multiple Myeloma
Cell culture
Cell Culture Techniques
Cells
Plasma Cells
Cell Line
Tumors
Cultured Cells
CD38 Antigens
B-Lymphocytes
Epstein-Barr Virus Nuclear Antigens
Cultured Tumor Cells
Plasmas
Chromosomes, Human, Pair 8
B-Cell Antigen Receptors
Passive Cutaneous Anaphylaxis
Transferrin Receptors

ASJC Scopus subject areas

  • Hematology

Cite this

Gazdar, A. F., Oie, H. K., Kirsch, I. R., & Hollis, G. F. (1986). Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. Blood, 67(6), 1542-1549.

Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. / Gazdar, A. F.; Oie, H. K.; Kirsch, I. R.; Hollis, G. F.

In: Blood, Vol. 67, No. 6, 1986, p. 1542-1549.

Research output: Contribution to journalArticle

Gazdar, AF, Oie, HK, Kirsch, IR & Hollis, GF 1986, 'Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene', Blood, vol. 67, no. 6, pp. 1542-1549.
Gazdar, A. F. ; Oie, H. K. ; Kirsch, I. R. ; Hollis, G. F. / Establishment and characterization of a human plasma cell myeloma culture having a rearranged cellular myc proto-oncogene. In: Blood. 1986 ; Vol. 67, No. 6. pp. 1542-1549.
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