Ethanol enhances GABA-induced 36Cl-influx in primary spinal cord cultured neurons

M. K. Ticku, P. Lowrimore, P. Lehoullier

Research output: Contribution to journalArticle

146 Scopus citations

Abstract

Ethanol has a pharmacological profile similar to other centrally acting drugs, which facilitate GABAergic transmission. GABA is known to produce its effects by increasing the conductance to Cl- ions. In this study, we have examined the effect of ethanol on GABA-induced 36Cl-influx in primary spinal cord cultured neurons. GABA produces a concentration-dependent and saturable effect on 36Cl-influx in these neurons. Ethanol potentiates the effect of GABA on 36Cl-influx in these neurons. GABA (20 μM) increased the 36Cl-influx by 75% over the basal value and in the presence of 50 mM ethanol, the observed increase was 142%. Eadie-Hoffstee analysis of the saturation curves indicated that ethanol decreases the Km value of GABA (10.6 μM to 4.2 μM) and also increases the Vmax. Besides potentiating the effect of GABA, ethanol also appears to have a direct effect in the absence of added GABA. These results suggest that ethanol enhances GABA-induced 36Cl-influx and indicate a role of GABAergic system in the actions of ethanol. These results also support the behavioral and electrophysiological studies, which have implicated GABA systems in the actions of ethanol. The potential mechanism(s) and the role of direct effect of ethanol is not clear at this time, but is currently being investigated.

Original languageEnglish (US)
Pages (from-to)123-126
Number of pages4
JournalBrain Research Bulletin
Volume17
Issue number1
DOIs
StatePublished - Jul 1986

Keywords

  • GABAergic transmission Ethanol Spinal cord cultured neurons Chloride ionophore

ASJC Scopus subject areas

  • Neuroscience(all)

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