Ets-1 and Ets-2 regulate the expression of MicroRNA-126 in endothelial cells

Tamia A. Harris, Munekazu Yamakuchi, Maiko Kondo, Peter Oettgen, Charles J. Lowenstein

Research output: Contribution to journalArticlepeer-review

103 Scopus citations

Abstract

Objective-: MicroRNA plays important roles in vascular biology, but the regulation of endothelial-specific microRNA is not well characterized. MicroRNA-126 (miR-126) is highly expressed in endothelial cells, and it regulates angiogenesis and vascular inflammation. Here we show that the transcription factors Ets-1 and Ets-2 regulate miR-126 expression. Methods and results-: A genomic region between-71 and-100 bp upstream of the miR-126 transcriptional start site is critical for transactivation of the gene containing miR-126. This genomic region contains a potential Ets binding site. Mutations within the Ets binding site block transactivation, and Ets-1 and Ets-2 interact with this critical genomic region. Knockdown of endogenous Ets-1 and Ets-2 decreases miR-126 expression. Finally, knockdown of miR-126 alters regulation of an Ets-1 target gene. Conclusion-: Taken together, these data show that the transcription factors Ets-1 and Ets-2 play a key role in controlling the expression of miR-126 and suggest that miR-126 may mediate some of their vascular effects.

Original languageEnglish (US)
Pages (from-to)1990-1997
Number of pages8
JournalArteriosclerosis, thrombosis, and vascular biology
Volume30
Issue number10
DOIs
StatePublished - Oct 2010

Keywords

  • endothelium
  • gene expression
  • immune system
  • microRNA
  • vascular biology

ASJC Scopus subject areas

  • Cardiology and Cardiovascular Medicine

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