Evaluation of a novel laparoscopic camera for characterization of renal ischemia in a porcine model using digital light processing (DLP) hyperspectral imaging

Ephrem O. Olweny, Yung K. Tan, Stephen Faddegon, Neil Jackson, Eleanor F. Wehner, Sara L. Best, Samuel K. Park, Abhas Thapa, Jeffrey A Cadeddu, Karel J. Zuzak

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Introduction: Digital light processing hyperspectral imaging (DLP® HSI) was adapted for use during laparoscopic surgery by coupling a conventional laparoscopic light guide with a DLP-based Agile Light source (OL 490, Optronic Laboratories, Orlando, FL), incorporating a 00 laparoscope, and a customized digital CCD camera (DVC, Austin, TX). The system was used to characterize renal ischemia in a porcine model. Methods: Laparoscopic DLP® HSI was performed in 2 groups of uni-nephric adult pigs that were each subjected to 90 minutes of warm ischemia. Group 1 (n=5) received tadalafil to mitigate ischemia/reperfusion injury while Group 2 (n=4) received no treatment. HSI-derived percentage of oxygenated hemoglobin (%HbO2) was plotted against time before, during and after ischemia. Serum creatinine measured pre-ischemia (D0), and on days 1, 3 and 7 post-ischemia, was correlated with %HbO2 using the Spearman's rank test. %HbO2 was compared for the groups using the Student's t-test. Results: Mean baseline and nadir %HbO2 for tadalafil vs. controls was 74% vs. 75% and 50% vs. 54% respectively; values did not significantly differ between the groups at any sampled time point. D0 creatinine was significantly lower for the tadalafil group, but creatinine at all other time points was similar between the groups. Baseline and nadir %HbO2 significantly correlated with D7 and D0 creatinine respectively in the tadalafil group alone. Conclusion: Laparoscopic DLP® HSI effectively characterizes renal ischemia, appears to be sensitive to changes in local blood flow, and is predictive of renal functional injury. Further parameterization and technological improvement is required before clinical incorporation.

Original languageEnglish (US)
Title of host publicationEmerging Digital Micromirror Device Based Systems and Applications IV
DOIs
StatePublished - Apr 16 2012
EventEmerging Digital Micromirror Device Based Systems and Applications IV - San Francisco, CA, United States
Duration: Jan 23 2012Jan 25 2012

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume8254
ISSN (Print)0277-786X

Other

OtherEmerging Digital Micromirror Device Based Systems and Applications IV
CountryUnited States
CitySan Francisco, CA
Period1/23/121/25/12

Keywords

  • Hemoglobins
  • Ischemia
  • Kidney
  • Laparoscopy
  • Light
  • Perfusion imaging
  • Phosphodiesterase inhibitors
  • Reperfusion injury
  • Spectrum analysis
  • Surgical procedures, minimally invasive

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

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