Evaluation of antigen-specific responses using in vitro enriched T cells

N. Jones, D. Agrawal, M. Elrefaei, A. Hanson, V. Novitsky, J. T. Wong, Huyen Cao

Research output: Contribution to journalArticle

29 Citations (Scopus)

Abstract

Antigen-specific lymphocytes are important in the immune response to viral infection. Peripheral blood mononuclear cells (PBMC) are traditionally used as a source of effector cells in most immunological studies. We described here the use of the bispecific monoclonal antibodies (BSMAB) anti CD3:CD8 (CD3,8) and anti CD3:CD4 (CD3,4B) to expand and selectively enrich CD4+ and CD8+ T cells populations, respectively. The expanded cells demonstrated >90% CD3+CD4+ or CD3+CD8+ by 14 days. We measured HIV- and CMV-specific responses of these subset-enriched T cell and found that sensitivity and specificity is similar or higher when compared to PBMC in various cellular immunology assays (CMI). Vbeta analysis of BSMAB-enriched cells demonstrated comparable repertoire to the parent PBMC. Although both CD45RAhi and CD45ROhi cell populations were expanded with the BSMAB, selective subset depletion demonstrated that the antigen-specific T cell responses were restricted to the initial CD45ROhi memory effector subgroup. In conclusion, BSMAB in vitro enrichment of T cells allows significant expansion of the cell population without loss of specificity. This technique of cell expansion permits studies of T cell subset function in situations where the initial cell source is scarce, and presents an alternative for viable and functional T cells in immunological assays.

Original languageEnglish (US)
Pages (from-to)139-147
Number of pages9
JournalJournal of Immunological Methods
Volume274
Issue number1-2
DOIs
StatePublished - Mar 1 2003
Externally publishedYes

Fingerprint

Bispecific Antibodies
T-Lymphocytes
Antigens
Blood Cells
T-Lymphocyte Subsets
Population
Virus Diseases
In Vitro Techniques
Allergy and Immunology
HIV
Lymphocytes
Sensitivity and Specificity

Keywords

  • Bispecific monoclonal antibody
  • CMI
  • T cell

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Jones, N., Agrawal, D., Elrefaei, M., Hanson, A., Novitsky, V., Wong, J. T., & Cao, H. (2003). Evaluation of antigen-specific responses using in vitro enriched T cells. Journal of Immunological Methods, 274(1-2), 139-147. https://doi.org/10.1016/S0022-1759(02)00510-0

Evaluation of antigen-specific responses using in vitro enriched T cells. / Jones, N.; Agrawal, D.; Elrefaei, M.; Hanson, A.; Novitsky, V.; Wong, J. T.; Cao, Huyen.

In: Journal of Immunological Methods, Vol. 274, No. 1-2, 01.03.2003, p. 139-147.

Research output: Contribution to journalArticle

Jones, N, Agrawal, D, Elrefaei, M, Hanson, A, Novitsky, V, Wong, JT & Cao, H 2003, 'Evaluation of antigen-specific responses using in vitro enriched T cells', Journal of Immunological Methods, vol. 274, no. 1-2, pp. 139-147. https://doi.org/10.1016/S0022-1759(02)00510-0
Jones N, Agrawal D, Elrefaei M, Hanson A, Novitsky V, Wong JT et al. Evaluation of antigen-specific responses using in vitro enriched T cells. Journal of Immunological Methods. 2003 Mar 1;274(1-2):139-147. https://doi.org/10.1016/S0022-1759(02)00510-0
Jones, N. ; Agrawal, D. ; Elrefaei, M. ; Hanson, A. ; Novitsky, V. ; Wong, J. T. ; Cao, Huyen. / Evaluation of antigen-specific responses using in vitro enriched T cells. In: Journal of Immunological Methods. 2003 ; Vol. 274, No. 1-2. pp. 139-147.
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