Evaluation of bacteriochlorophyll-reconstituted low-density lipoprotein nanoparticles for photodynamic therapy efficacy in vivo

Diane E. Marotta, Weiguo Cao, E. Paul Wileyto, Hui Li, Ian Corbin, Elizabeth Rickter, Jerry D. Glickson, Britton Chance, Gang Zheng, Theresa M. Busch

Research output: Contribution to journalArticle

32 Scopus citations

Abstract

Aim: To evaluate the novel nanoparticle reconstituted bacteriochlorin e6 bisoleate low-density lipoprotein (r-Bchl-BOA-LDL) for its efficacy as a photodynamic therapy agent delivery system in xenografts of human hepatoblastoma G2 (HepG2) tumors. Materials & methods: Bchl-BOA was encapsulated in the nanoparticle low-density lipoprotein (LDL), a native particle whose receptor's overexpression is a cancer signature for a number of neoplasms. Evaluation of r-Bchl-BOA-LDL as a potential photosensitizer was performed using a tumor response and foot response assay. Results & discussion: When compared with controls, tumor regrowth was significantly delayed at injected murine doses of 2 μmole/kg r-Bchl-BOA-LDL after illumination at fluences of 125, 150 or 175 J/cm2. Foot response assays showed that although normal tissue toxicity accompanied the higher fluences it was significantly reduced at the lowest fluence tested. Conclusion: This research demonstrates that r-Bchl-BOA-LDL is an effective photosensitizer and a promising candidate for further investigation.

Original languageEnglish (US)
Pages (from-to)475-487
Number of pages13
JournalNanomedicine
Volume6
Issue number3
DOIs
StatePublished - Apr 1 2011

Keywords

  • bacteriochlorophyll
  • low-density lipoprotein
  • nanoparticle
  • photodynamic therapy
  • xenograft hepatoma

ASJC Scopus subject areas

  • Bioengineering
  • Medicine (miscellaneous)
  • Biomedical Engineering
  • Materials Science(all)

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    Marotta, D. E., Cao, W., Wileyto, E. P., Li, H., Corbin, I., Rickter, E., Glickson, J. D., Chance, B., Zheng, G., & Busch, T. M. (2011). Evaluation of bacteriochlorophyll-reconstituted low-density lipoprotein nanoparticles for photodynamic therapy efficacy in vivo. Nanomedicine, 6(3), 475-487. https://doi.org/10.2217/nnm.11.8