Evaluation of monoclonal antibodies for the development of breast cancer immunotoxins

M. J. Bjorn, D. Ring, A. Frankel

Research output: Contribution to journalArticle

101 Citations (Scopus)

Abstract

Eight-five antibodies recognizing breast cancer-selective antigens were conjugated to ricin toxin A-chain using a disulfide linkage. The cytotoxicities of the resulting immunotoxins were determined on breast cancer cells and normal human fibroblasts. Twenty-four antibodies formed immunotoxins that were toxic to at least one breast cancer cell line at concentrations of 10 nM or less but were nontoxic to human fibroblast lines used as negative controls. Some of the breast tumor-selective immunotoxins were as toxic as a conjugate between monoclonal anti-transferrin receptor and ricin toxin A-chain (50% inhibition of cellular protein synthesis at approximately 0.1 nM). Another set of four immunotoxins were indiscriminately toxic to human breast tumor cel lines, two human fibroblast cell lines, and a human lymphoblastoid line. Several of the antibodies the toxin conjugates of which specifically killed breast cancer cell lines may be useful in cancer therapy, since they show a wide range of binding to individual breast tumors and cell lines and a limited range of binding to normal tissue types.

Original languageEnglish (US)
Pages (from-to)1214-1221
Number of pages8
JournalCancer Research
Volume45
Issue number3
StatePublished - 1985

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Immunotoxins
Monoclonal Antibodies
Breast Neoplasms
Poisons
Ricin
Fibroblasts
Cell Line
Transferrin Receptors
Antibodies
Tumor Cell Line
Disulfides
Antigens

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Evaluation of monoclonal antibodies for the development of breast cancer immunotoxins. / Bjorn, M. J.; Ring, D.; Frankel, A.

In: Cancer Research, Vol. 45, No. 3, 1985, p. 1214-1221.

Research output: Contribution to journalArticle

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