Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150

Peter Papathanasiou, Joanne L. Attema, Holger Karsunky, Xu Jian, Stephen T. Smale, Irving L. Weissman

Research output: Contribution to journalArticle

35 Citations (Scopus)

Abstract

Blood is a tissue with a high cell turnover rate that is constantly being replenished by bone marrow hematopoietic stem cells (HSCs) seeded during fetal ontogeny from the liver. Here we show that the long-term (LT) reconstituting subset of cKit+Thy1.1(lo)Lin(-/lo)Sca1+Flk2- HSCs is CD150+. HSCs sourced from the fetal liver show LT, multilineage engraftment from E14.5 onward, and the CD150 cell surface molecule can readily substitute Thy1.1 as a positive marker of LT-HSCs in this tissue. From both fetal liver and adult bone marrow, cKit+Thy1.1(lo)Lin(-/lo) Sca1+Flk2- CD1501 cells exhibit robust LT competitive engraftment, self-renewal, multilineage differentiation capacity, and an accessible chromatin configuration consistent with high expression of erythroid/megakaryoid genes in purified cell subsets. Our data show that, with appropriate combinations of cell surface markers, stem cells can be accurately isolated to high purity and characterized. This is important for the clarification of lineage relationships and the identification of bona fide regulators of stem cell self-renewal and differentiation both in normal and neoplastic tissues.

Original languageEnglish (US)
Pages (from-to)2498-2508
Number of pages11
JournalStem Cells
Volume27
Issue number10
DOIs
StatePublished - Oct 2009

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Hematopoietic Stem Cells
Liver
Bone Marrow
Chromatin
Stem Cells
Genes

Keywords

  • CD150
  • Fetal liver
  • Hematopoietic stem cells

ASJC Scopus subject areas

  • Cell Biology
  • Developmental Biology
  • Molecular Medicine

Cite this

Papathanasiou, P., Attema, J. L., Karsunky, H., Jian, X., Smale, S. T., & Weissman, I. L. (2009). Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150. Stem Cells, 27(10), 2498-2508. https://doi.org/10.1002/stem.170

Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150. / Papathanasiou, Peter; Attema, Joanne L.; Karsunky, Holger; Jian, Xu; Smale, Stephen T.; Weissman, Irving L.

In: Stem Cells, Vol. 27, No. 10, 10.2009, p. 2498-2508.

Research output: Contribution to journalArticle

Papathanasiou, P, Attema, JL, Karsunky, H, Jian, X, Smale, ST & Weissman, IL 2009, 'Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150', Stem Cells, vol. 27, no. 10, pp. 2498-2508. https://doi.org/10.1002/stem.170
Papathanasiou P, Attema JL, Karsunky H, Jian X, Smale ST, Weissman IL. Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150. Stem Cells. 2009 Oct;27(10):2498-2508. https://doi.org/10.1002/stem.170
Papathanasiou, Peter ; Attema, Joanne L. ; Karsunky, Holger ; Jian, Xu ; Smale, Stephen T. ; Weissman, Irving L. / Evaluation of the long-term reconstituting subset of hematopoietic stem cells with CD150. In: Stem Cells. 2009 ; Vol. 27, No. 10. pp. 2498-2508.
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