Evaluation of the matrix effect of thermophilic anaerobic digestion on inactivation of infectious laryngotracheitis virus using real-time PCR and viral cell culture

Tiejun Gao; Evelyn Bowlby; Yupin Tong; John T.Y. Wu; Lester Wong; Robert J. Tower; Xiaoli Pang; Xiaomei Li

doi:10.1016/j.biortech.2012.01.166

Evaluation of the matrix effect of thermophilic anaerobic digestion on inactivation of infectious laryngotracheitis virus using real-time PCR and viral cell culture

Tiejun Gao, Evelyn Bowlby, Yupin Tong, John T.Y. Wu, Lester Wong, Robert J. Tower, Xiaoli Pang, Xiaomei Li

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

The matrix effect of the thermophilic anaerobic digestion (TAD) process on inactivation of infectious laryngotracheitis virus (ILTV) was evaluated. Viral cell culture and real-time PCR were used for assessing removal of the viral infectivity and degradation of viral DNA, respectively. Results showed that the TAD-derived matrix alone can inactivate the virus and destroy the nucleic acid helix core of ILTV in a time-and- dose-dependent manner. No cytopathogenic effect (CPE) was observed in the cells exposed to ILTV pre-treated with TAD matrix for 1.5. h in experiment 1 and for 16. h in experiment 2. There was a significant statistical difference between TAD matrix treated and non-treated cultures (p<. 0.001, Chi-test). Amplifiable ILT viral DNA was reduced 2.27 log by 1.5. h-treatment and was not present by 16. h-treatment with TAD matrix, indicating complete viral DNA fragmentation. The TAD process is an environmentally friendly way for disposing of poultry biowaste and carcasses.

Original language	English (US)
Pages (from-to)	692-696
Number of pages	5
Journal	Bioresource Technology
Volume	110
DOIs	https://doi.org/10.1016/j.biortech.2012.01.166
State	Published - Apr 2012
Externally published	Yes

Keywords

Anaerobic digestion
Biowaste
Inactivation
Infectious laryngotracheitis virus
Poultry
Thermophilic

ASJC Scopus subject areas

Bioengineering
Environmental Engineering
Renewable Energy, Sustainability and the Environment
Waste Management and Disposal

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10.1016/j.biortech.2012.01.166

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title = "Evaluation of the matrix effect of thermophilic anaerobic digestion on inactivation of infectious laryngotracheitis virus using real-time PCR and viral cell culture",

abstract = "The matrix effect of the thermophilic anaerobic digestion (TAD) process on inactivation of infectious laryngotracheitis virus (ILTV) was evaluated. Viral cell culture and real-time PCR were used for assessing removal of the viral infectivity and degradation of viral DNA, respectively. Results showed that the TAD-derived matrix alone can inactivate the virus and destroy the nucleic acid helix core of ILTV in a time-and- dose-dependent manner. No cytopathogenic effect (CPE) was observed in the cells exposed to ILTV pre-treated with TAD matrix for 1.5. h in experiment 1 and for 16. h in experiment 2. There was a significant statistical difference between TAD matrix treated and non-treated cultures (p<. 0.001, Chi-test). Amplifiable ILT viral DNA was reduced 2.27 log by 1.5. h-treatment and was not present by 16. h-treatment with TAD matrix, indicating complete viral DNA fragmentation. The TAD process is an environmentally friendly way for disposing of poultry biowaste and carcasses.",

keywords = "Anaerobic digestion, Biowaste, Inactivation, Infectious laryngotracheitis virus, Poultry, Thermophilic",

author = "Tiejun Gao and Evelyn Bowlby and Yupin Tong and Wu, {John T.Y.} and Lester Wong and Tower, {Robert J.} and Xiaoli Pang and Xiaomei Li",

year = "2012",

month = apr,

doi = "10.1016/j.biortech.2012.01.166",

language = "English (US)",

volume = "110",

pages = "692--696",

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AU - Gao, Tiejun

AU - Bowlby, Evelyn

AU - Tong, Yupin

AU - Wu, John T.Y.

AU - Wong, Lester

AU - Tower, Robert J.

AU - Pang, Xiaoli

AU - Li, Xiaomei

PY - 2012/4

Y1 - 2012/4

N2 - The matrix effect of the thermophilic anaerobic digestion (TAD) process on inactivation of infectious laryngotracheitis virus (ILTV) was evaluated. Viral cell culture and real-time PCR were used for assessing removal of the viral infectivity and degradation of viral DNA, respectively. Results showed that the TAD-derived matrix alone can inactivate the virus and destroy the nucleic acid helix core of ILTV in a time-and- dose-dependent manner. No cytopathogenic effect (CPE) was observed in the cells exposed to ILTV pre-treated with TAD matrix for 1.5. h in experiment 1 and for 16. h in experiment 2. There was a significant statistical difference between TAD matrix treated and non-treated cultures (p<. 0.001, Chi-test). Amplifiable ILT viral DNA was reduced 2.27 log by 1.5. h-treatment and was not present by 16. h-treatment with TAD matrix, indicating complete viral DNA fragmentation. The TAD process is an environmentally friendly way for disposing of poultry biowaste and carcasses.

AB - The matrix effect of the thermophilic anaerobic digestion (TAD) process on inactivation of infectious laryngotracheitis virus (ILTV) was evaluated. Viral cell culture and real-time PCR were used for assessing removal of the viral infectivity and degradation of viral DNA, respectively. Results showed that the TAD-derived matrix alone can inactivate the virus and destroy the nucleic acid helix core of ILTV in a time-and- dose-dependent manner. No cytopathogenic effect (CPE) was observed in the cells exposed to ILTV pre-treated with TAD matrix for 1.5. h in experiment 1 and for 16. h in experiment 2. There was a significant statistical difference between TAD matrix treated and non-treated cultures (p<. 0.001, Chi-test). Amplifiable ILT viral DNA was reduced 2.27 log by 1.5. h-treatment and was not present by 16. h-treatment with TAD matrix, indicating complete viral DNA fragmentation. The TAD process is an environmentally friendly way for disposing of poultry biowaste and carcasses.

KW - Anaerobic digestion

KW - Biowaste

KW - Inactivation

KW - Infectious laryngotracheitis virus

KW - Poultry

KW - Thermophilic

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Evaluation of the matrix effect of thermophilic anaerobic digestion on inactivation of infectious laryngotracheitis virus using real-time PCR and viral cell culture

Abstract

Keywords

ASJC Scopus subject areas

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