Evaluation of the relative cytotoxic effects of anticancer agents in serum-supplemented versus serum-free media using a tetrazolium colorimetric assay

Chun Ming Tsai, Reury Perng Perng, Kuo Ting Chang, David Venzon, Adi F. Gazdar

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Most cell culture and in vitro drug sensitivity assays utilize serum-supplemented media (SSM). However, fully defined serum-free media (SFM) offer several advantages and are being used increasingly for initiation and maintenance of cell cultures. Because serum inhibits the in vitro cytotoxicity of certain antineoplastic agents, we investigated the inter-relationships between medium type, cell proliferation and cytotoxic effect. Twenty-four human lung cancer cell lines were tested with nine anticancer agents in both media types. A semi-automated tetrazolium (MTT) colorimetric assay was used for assaying cell survival. Cell lines initiated and maintained in SFM preferentially proliferated in that medium type or proliferated equally well in both media types. In contrast, cell lines established in SSM varied considerably in their medium of preference. There appeared to be a direct correlation or trend between cell proliferative rate and cytotoxicity of all drugs with the possible exceptions of methotrexate and carmustine. In general, the cell lines were more sensitive to anticancer agents when they were exposed in the culture medium in which they preferentially proliferated. Therefore, to determine the influence of culture media on cytotoxicity, we analyzed the data only from lines that replicated equally efficiently in both media. After correction for cell proliferative rate, SSM had a negative effect on the cytotoxic action of some drugs (especially methotrexate, 5-fluorouracil and, to a lesser extent, mitomycin-C). Our results demonstrate that fully defined SFM may be suitable for initiating cell lines and for use in in vitro cytotoxicity assays for selection of individualized therapy or for screening of new anti-neoplastic agents, and thus may increase the number of antineoplastic agents that can be tested satisfactorily.

Original languageEnglish (US)
Pages (from-to)91-97
Number of pages7
JournalJapanese Journal of Cancer Research
Volume87
Issue number1
StatePublished - Jan 1996

Fingerprint

Serum-Free Culture Media
Antineoplastic Agents
Cell Line
Serum
Methotrexate
Culture Media
Cell Culture Techniques
Pharmaceutical Preparations
Carmustine
Mitomycin
Fluorouracil
Lung Neoplasms
Cell Survival
Maintenance
Cell Proliferation
In Vitro Techniques

Keywords

  • Anticancer agent
  • Drug testing
  • Lung cancer
  • Serum-free medium

ASJC Scopus subject areas

  • Cancer Research
  • Oncology

Cite this

Evaluation of the relative cytotoxic effects of anticancer agents in serum-supplemented versus serum-free media using a tetrazolium colorimetric assay. / Tsai, Chun Ming; Perng, Reury Perng; Chang, Kuo Ting; Venzon, David; Gazdar, Adi F.

In: Japanese Journal of Cancer Research, Vol. 87, No. 1, 01.1996, p. 91-97.

Research output: Contribution to journalArticle

Tsai, Chun Ming ; Perng, Reury Perng ; Chang, Kuo Ting ; Venzon, David ; Gazdar, Adi F. / Evaluation of the relative cytotoxic effects of anticancer agents in serum-supplemented versus serum-free media using a tetrazolium colorimetric assay. In: Japanese Journal of Cancer Research. 1996 ; Vol. 87, No. 1. pp. 91-97.
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