Evaluation of triple quantum-filtered 23Na NMR spectroscopy in the in situ rat liver

Viswanathan Seshan, A. Dean Sherry, Navin Bansal

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Triple quantum (TQ)-filtered 23Na NMR spectroscopy and the shift reagent, TmDOTP5-, have been used to evaluate the contributions of intra- (Na(i)+) and extracellular (Na(e)+) sodium to the TQ-filtered signal in the rat liver, in situ. Na(e)+ contributed significantly to the total TQ- filtered signal in live animals, and the intensity of this signal did not change postmortem. The TQ-filtered Na(i)+ signal increased by approximately 380% over a period of 1 h postmortem, whereas the single quantum (SQ) Na(i)+ increased by 90%. The constancy of the TQ-filtered Na(e)+ signal indicates that changes in total TQ-filtered 23Na signal intensity in liver (without a shift reagent) may accurately reflect changes in TQ-filtered Na(i)+ signal intensity. The large percent increase in the TQ-filtered Na(i)+ signal as compared to the SQ signal suggests that the fraction of Na(i)+ interacting with macromolecules increases after death.

Original languageEnglish (US)
Pages (from-to)821-827
Number of pages7
JournalMagnetic Resonance in Medicine
Volume38
Issue number5
DOIs
StatePublished - Nov 1997

Fingerprint

Magnetic Resonance Spectroscopy
Postmortem Changes
Liver
Sodium

Keywords

  • Na MRS
  • Liver
  • Multiple quantum NMR
  • Shift reagent

ASJC Scopus subject areas

  • Radiology Nuclear Medicine and imaging
  • Radiological and Ultrasound Technology

Cite this

Evaluation of triple quantum-filtered 23Na NMR spectroscopy in the in situ rat liver. / Seshan, Viswanathan; Sherry, A. Dean; Bansal, Navin.

In: Magnetic Resonance in Medicine, Vol. 38, No. 5, 11.1997, p. 821-827.

Research output: Contribution to journalArticle

@article{e227848e87c34fe79a90193a7cb83bec,
title = "Evaluation of triple quantum-filtered 23Na NMR spectroscopy in the in situ rat liver",
abstract = "Triple quantum (TQ)-filtered 23Na NMR spectroscopy and the shift reagent, TmDOTP5-, have been used to evaluate the contributions of intra- (Na(i)+) and extracellular (Na(e)+) sodium to the TQ-filtered signal in the rat liver, in situ. Na(e)+ contributed significantly to the total TQ- filtered signal in live animals, and the intensity of this signal did not change postmortem. The TQ-filtered Na(i)+ signal increased by approximately 380{\%} over a period of 1 h postmortem, whereas the single quantum (SQ) Na(i)+ increased by 90{\%}. The constancy of the TQ-filtered Na(e)+ signal indicates that changes in total TQ-filtered 23Na signal intensity in liver (without a shift reagent) may accurately reflect changes in TQ-filtered Na(i)+ signal intensity. The large percent increase in the TQ-filtered Na(i)+ signal as compared to the SQ signal suggests that the fraction of Na(i)+ interacting with macromolecules increases after death.",
keywords = "Na MRS, Liver, Multiple quantum NMR, Shift reagent",
author = "Viswanathan Seshan and Sherry, {A. Dean} and Navin Bansal",
year = "1997",
month = "11",
doi = "10.1002/mrm.1910380519",
language = "English (US)",
volume = "38",
pages = "821--827",
journal = "Magnetic Resonance in Medicine",
issn = "0740-3194",
publisher = "John Wiley and Sons Inc.",
number = "5",

}

TY - JOUR

T1 - Evaluation of triple quantum-filtered 23Na NMR spectroscopy in the in situ rat liver

AU - Seshan, Viswanathan

AU - Sherry, A. Dean

AU - Bansal, Navin

PY - 1997/11

Y1 - 1997/11

N2 - Triple quantum (TQ)-filtered 23Na NMR spectroscopy and the shift reagent, TmDOTP5-, have been used to evaluate the contributions of intra- (Na(i)+) and extracellular (Na(e)+) sodium to the TQ-filtered signal in the rat liver, in situ. Na(e)+ contributed significantly to the total TQ- filtered signal in live animals, and the intensity of this signal did not change postmortem. The TQ-filtered Na(i)+ signal increased by approximately 380% over a period of 1 h postmortem, whereas the single quantum (SQ) Na(i)+ increased by 90%. The constancy of the TQ-filtered Na(e)+ signal indicates that changes in total TQ-filtered 23Na signal intensity in liver (without a shift reagent) may accurately reflect changes in TQ-filtered Na(i)+ signal intensity. The large percent increase in the TQ-filtered Na(i)+ signal as compared to the SQ signal suggests that the fraction of Na(i)+ interacting with macromolecules increases after death.

AB - Triple quantum (TQ)-filtered 23Na NMR spectroscopy and the shift reagent, TmDOTP5-, have been used to evaluate the contributions of intra- (Na(i)+) and extracellular (Na(e)+) sodium to the TQ-filtered signal in the rat liver, in situ. Na(e)+ contributed significantly to the total TQ- filtered signal in live animals, and the intensity of this signal did not change postmortem. The TQ-filtered Na(i)+ signal increased by approximately 380% over a period of 1 h postmortem, whereas the single quantum (SQ) Na(i)+ increased by 90%. The constancy of the TQ-filtered Na(e)+ signal indicates that changes in total TQ-filtered 23Na signal intensity in liver (without a shift reagent) may accurately reflect changes in TQ-filtered Na(i)+ signal intensity. The large percent increase in the TQ-filtered Na(i)+ signal as compared to the SQ signal suggests that the fraction of Na(i)+ interacting with macromolecules increases after death.

KW - Na MRS

KW - Liver

KW - Multiple quantum NMR

KW - Shift reagent

UR - http://www.scopus.com/inward/record.url?scp=0030666558&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0030666558&partnerID=8YFLogxK

U2 - 10.1002/mrm.1910380519

DO - 10.1002/mrm.1910380519

M3 - Article

C2 - 9358457

AN - SCOPUS:0030666558

VL - 38

SP - 821

EP - 827

JO - Magnetic Resonance in Medicine

JF - Magnetic Resonance in Medicine

SN - 0740-3194

IS - 5

ER -