TY - JOUR
T1 - Evidence for a 40,000 dalton species of plastocyanin as the active component
AU - Siegelman, M. H.
AU - Rasched, I.
AU - Böger, P.
N1 - Funding Information:
Acknowledgements The authors are grateful to Dr.P.Kroneck for the EPR measure-~ents and K.J.Kunert for his valuable contributions in assay techniques and preparation of the plastocyanins. This study was supported by the Deutsche Forschungsgemeinschaft through the Sonderforschungsbereich 138.
PY - 1975/8/18
Y1 - 1975/8/18
N2 - Evidence is presented for a molecular weight of 40,000 daltons for plastocyanin from spinach and Scenedesmus employing SDS polyacrylamide gel analysis. We propose that this component is the tetramer of a 10,000 dalton species, the component found after reduction with dithiothreitol. The tetramer is virtually absent in the apoprotein but reappears on reconstitution by copper sulfate titration. Tetramer, but neither dimer nor monomer produced by various procedures given here, demonstrates activity and EPR-active copper strongly suggesting it represents the native state of the protein.
AB - Evidence is presented for a molecular weight of 40,000 daltons for plastocyanin from spinach and Scenedesmus employing SDS polyacrylamide gel analysis. We propose that this component is the tetramer of a 10,000 dalton species, the component found after reduction with dithiothreitol. The tetramer is virtually absent in the apoprotein but reappears on reconstitution by copper sulfate titration. Tetramer, but neither dimer nor monomer produced by various procedures given here, demonstrates activity and EPR-active copper strongly suggesting it represents the native state of the protein.
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U2 - 10.1016/S0006-291X(75)80392-5
DO - 10.1016/S0006-291X(75)80392-5
M3 - Article
C2 - 1052434
AN - SCOPUS:0016710395
SN - 0006-291X
VL - 65
SP - 1456
EP - 1463
JO - Biochemical and Biophysical Research Communications
JF - Biochemical and Biophysical Research Communications
IS - 4
ER -