TY - JOUR
T1 - Evidence for H+ secretion by the in vivo canine gallbladder
AU - Rege, Robert V
AU - Moore, Edward W.
N1 - Funding Information:
Received March 6, 1985. Accepted June 23, 1986. Address requests for reprints to: Dr. Edward W. Moore, Box 711, MCV Station, Medical College of Virginia, Richmond, Virginia 23298. This work was supported by grant AM 32130 from the National Institute of Arthritis, Diabetes, Digestive and Kidney Diseases, National Institutes of Health. This work was presented in part at the annual meeting of the American Association for the Study of Liver Diseases, Chicago, Illinois, November 1984 and was published in preliminary form in Hepatology 1984;4:1079. The authors gratefully acknowledge the able technical assistance of Kelli Mosher, Inge Moore, and Fran Keith. 0 1987 by the American Gastroenterological Association 0016-5085/87/$3.50
PY - 1987/2
Y1 - 1987/2
N2 - In humans and most other species, a decline in pH of gallbladder contents occurs during the concentration of bile. Recent in vitro studies in rabbit, guinea pig, and Necturus gallbladders have strongly suggested mucosal H+ secretion during sodium reabsorption, presumably representing a Na+ H+ exchange. The present in vivo studies are the first attempt to determine whether H+ secretion by the gallbladder can be demonstrated in the living animal. Gallbladder bile was obtained from 27 anesthetized dogs after 12-24-h fasts; 12 samples of common duct bile were also obtained in 3 dogs during variable taurocholate infusion. In common duct bile, observed ranges were as follows: pH, 7.3-7.85; CO2 partial pressure (Pco2), 21-32 mmHg; total CO2 concentration ([TCO2]), 16.4-41.4 mM; total bile salt concentration ([TBS]), 16-93 mM; and [Na], 153-192 mM. In gallbladder bile, respective ranges were as follows: pH, 5.72-7.29; Pco2, 36-101 mmHg; [TCO2], 1.21-15.5 mM; [TBS], 150-305 mM; and [Na], 199-266 mM. In all samples [Na] was linearly related to [TBS]. Carbon dioxide partial pressure increased from a mean of 27.3 mmHg in common duct bile to >100 mmHg in gallbladder bile at [TBS] = 180 mM, then declined to ~36 mmHg as [TBS] increased to >300 mM. Peak Pco2 occurred at pH ~6.4-6.6, then declined as pH decreased to ~5.7. Bile to plasma Pco2 ratios increased from a mean of 1.08 in common duct samples to >2.0 in gallbladder samples at pH ~6.3, then declined to ~1.0 in fully concentrated bile. If the high Pco2 values in bile were solely due to tissue CO2 production, a sustained increase in Pco2 throughout Na+ reabsorption might be expected. The results strongly suggest H+ secretion (HCO3- neutralization), as peak Pco2 occurred when [TBS] was only about 180 mM, long before sodium absorption was complete. It is hypothesized that H+ secretion may have important favorable effects on calcium lithogenicity, reducing the likelihood of the formation of CaCO3- containing gallstones.
AB - In humans and most other species, a decline in pH of gallbladder contents occurs during the concentration of bile. Recent in vitro studies in rabbit, guinea pig, and Necturus gallbladders have strongly suggested mucosal H+ secretion during sodium reabsorption, presumably representing a Na+ H+ exchange. The present in vivo studies are the first attempt to determine whether H+ secretion by the gallbladder can be demonstrated in the living animal. Gallbladder bile was obtained from 27 anesthetized dogs after 12-24-h fasts; 12 samples of common duct bile were also obtained in 3 dogs during variable taurocholate infusion. In common duct bile, observed ranges were as follows: pH, 7.3-7.85; CO2 partial pressure (Pco2), 21-32 mmHg; total CO2 concentration ([TCO2]), 16.4-41.4 mM; total bile salt concentration ([TBS]), 16-93 mM; and [Na], 153-192 mM. In gallbladder bile, respective ranges were as follows: pH, 5.72-7.29; Pco2, 36-101 mmHg; [TCO2], 1.21-15.5 mM; [TBS], 150-305 mM; and [Na], 199-266 mM. In all samples [Na] was linearly related to [TBS]. Carbon dioxide partial pressure increased from a mean of 27.3 mmHg in common duct bile to >100 mmHg in gallbladder bile at [TBS] = 180 mM, then declined to ~36 mmHg as [TBS] increased to >300 mM. Peak Pco2 occurred at pH ~6.4-6.6, then declined as pH decreased to ~5.7. Bile to plasma Pco2 ratios increased from a mean of 1.08 in common duct samples to >2.0 in gallbladder samples at pH ~6.3, then declined to ~1.0 in fully concentrated bile. If the high Pco2 values in bile were solely due to tissue CO2 production, a sustained increase in Pco2 throughout Na+ reabsorption might be expected. The results strongly suggest H+ secretion (HCO3- neutralization), as peak Pco2 occurred when [TBS] was only about 180 mM, long before sodium absorption was complete. It is hypothesized that H+ secretion may have important favorable effects on calcium lithogenicity, reducing the likelihood of the formation of CaCO3- containing gallstones.
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U2 - 10.1016/0016-5085(87)90118-1
DO - 10.1016/0016-5085(87)90118-1
M3 - Article
C2 - 3098620
AN - SCOPUS:0023065344
SN - 0016-5085
VL - 92
SP - 281
EP - 289
JO - Gastroenterology
JF - Gastroenterology
IS - 2
ER -