@article{0cc2b6aa08664aeeba57c4ec8eedc2f7,
title = "Evidence for immunoreactive neurotensin in dog intestinal mucosa",
abstract = "Discrete cells containing neurotensin, as shown by immunofluorescence, have been observed in the lower portion of the dog ileum. This implies that neurotensin may be synthesized in the small intestine and may be involved in local regulation of intestinal functions. Neurotensin is a peptide characterized originally in the hypothalamus.",
author = "L. Orci and O. Baetens and C. Rufener and M. Brown and W. Vale and R. Guillemin",
note = "Funding Information: Fresh samples of gastrointestinal mucosa (cardia, fundus, body and antrum of the stomach, duodenum, jejunum, ileum and colon) and pancreas were taken from dogs . The tissues were fixed in Bouin's solution (2 .2Z picric acid, 10Z formaldehyde and 5~ acetic acid), dehydrated and embedded in paraffin . The indirect lmmunofluorescence method of Coons et al . (10) was applied using deparaffinlzed 5 v sections . Rabbit antineurotensin prepared by one of us (M .B .) (11) was applied as the first antibody (1 :10 dilution) and fluorescein-labeled sheep anti-rabbit IgG as the second antibody . The specificity of the immunofluorescent reaction was ascertained by studying the inhibition of the reaction by homologous antigen (synthetic neurotensin 50 ug/ml) and heterologous antigens (g ucagon, GLI, i .e ., gut *Supported by a Grant (No . 3 .553 .75) from the Fonds National Suisse de 1a Recherche Scientifique and, at The Salk Institute, by research Grants Nos . HD-09690, AM-16707 and AM-18811 from the National Institutes of Health and by research Grant No . 1-411 from the National Foundation .",
year = "1976",
month = aug,
day = "15",
doi = "10.1016/0024-3205(76)90236-8",
language = "English (US)",
volume = "19",
pages = "559--561",
journal = "Life Sciences",
issn = "0024-3205",
publisher = "Elsevier Inc.",
number = "4",
}