Evidence for inactivation of DNA repair in frozen and thawed mammalian cells

H. Slor, T. Lev-Sobe, E. C. Friedberg

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

A variety of cell strains and lines were frozen and thawed by conventional techniques for cell storage. Following thawing, extracts of cells were prepared and incubated with UV-irradiated E. coli DNA. Thymine dimer excision activity present in extracts of unfrozen cells was lost in extracts of recently thawed cells. The ability to exercise dimers was restored after about 40 h post-thawing, but the recovery was inhibited if cells were cultured in the presence of puromycin. Correlating with the loss of dimer excising activity there was a reduced cell viability as measured by trypan blue dye exclusion.

Original languageEnglish (US)
Pages (from-to)137-145
Number of pages9
JournalMutation Research - Fundamental and Molecular Mechanisms of Mutagenesis
Volume45
Issue number1
DOIs
StatePublished - Oct 1977

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ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Health, Toxicology and Mutagenesis

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