Evidence for orientation-conserved transfer in the TCA cycle in Saccharomyces cerevisiae: 13C NMR studies

B. Sumegi, A. D. Sherry, C. R. Malloy, P. A. Srere

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

When [4-13C]glutamate is incubated with yeast cells, the [13C]aspartate formed shows a [2-13C]/[3-13C] ratio that is greater than 1. If the conversion occurs through the Krebs TCA cycle with the symmetrical intermediates succinate and fumarate, the ratio is expected to be 1. The addition of fluoroacetate to the incubation increases the ratio further. When the mutants Aco1- or CS1- are used, little or no aspartate is formed. When [2-13C]acetate is incubated with yeast, the C2-/C3-aspartate ratio is 1.5 at 2 min and decreases to 1.0 after 10 min of incubation. These results indicate that orientation-conserved transfer occurs at the succinate thiokinase and succinate dehydrogenase steps of the Krebs TCA cycle.

Original languageEnglish (US)
Pages (from-to)12725-12729
Number of pages5
JournalBiochemistry
Volume32
Issue number47
DOIs
StatePublished - 1993

Fingerprint

Aspartic Acid
Yeast
Saccharomyces cerevisiae
Citric Acid Cycle
Nuclear magnetic resonance
Succinic Acid
Fluoroacetates
Yeasts
Fumarates
Succinate Dehydrogenase
Glutamic Acid
Acetates
Cells
Carbon-13 Magnetic Resonance Spectroscopy

ASJC Scopus subject areas

  • Biochemistry

Cite this

Evidence for orientation-conserved transfer in the TCA cycle in Saccharomyces cerevisiae : 13C NMR studies. / Sumegi, B.; Sherry, A. D.; Malloy, C. R.; Srere, P. A.

In: Biochemistry, Vol. 32, No. 47, 1993, p. 12725-12729.

Research output: Contribution to journalArticle

@article{b597c9afc580446a997c5442c8018269,
title = "Evidence for orientation-conserved transfer in the TCA cycle in Saccharomyces cerevisiae: 13C NMR studies",
abstract = "When [4-13C]glutamate is incubated with yeast cells, the [13C]aspartate formed shows a [2-13C]/[3-13C] ratio that is greater than 1. If the conversion occurs through the Krebs TCA cycle with the symmetrical intermediates succinate and fumarate, the ratio is expected to be 1. The addition of fluoroacetate to the incubation increases the ratio further. When the mutants Aco1- or CS1- are used, little or no aspartate is formed. When [2-13C]acetate is incubated with yeast, the C2-/C3-aspartate ratio is 1.5 at 2 min and decreases to 1.0 after 10 min of incubation. These results indicate that orientation-conserved transfer occurs at the succinate thiokinase and succinate dehydrogenase steps of the Krebs TCA cycle.",
author = "B. Sumegi and Sherry, {A. D.} and Malloy, {C. R.} and Srere, {P. A.}",
year = "1993",
doi = "10.1021/bi00210a022",
language = "English (US)",
volume = "32",
pages = "12725--12729",
journal = "Biochemistry",
issn = "0006-2960",
publisher = "American Chemical Society",
number = "47",

}

TY - JOUR

T1 - Evidence for orientation-conserved transfer in the TCA cycle in Saccharomyces cerevisiae

T2 - 13C NMR studies

AU - Sumegi, B.

AU - Sherry, A. D.

AU - Malloy, C. R.

AU - Srere, P. A.

PY - 1993

Y1 - 1993

N2 - When [4-13C]glutamate is incubated with yeast cells, the [13C]aspartate formed shows a [2-13C]/[3-13C] ratio that is greater than 1. If the conversion occurs through the Krebs TCA cycle with the symmetrical intermediates succinate and fumarate, the ratio is expected to be 1. The addition of fluoroacetate to the incubation increases the ratio further. When the mutants Aco1- or CS1- are used, little or no aspartate is formed. When [2-13C]acetate is incubated with yeast, the C2-/C3-aspartate ratio is 1.5 at 2 min and decreases to 1.0 after 10 min of incubation. These results indicate that orientation-conserved transfer occurs at the succinate thiokinase and succinate dehydrogenase steps of the Krebs TCA cycle.

AB - When [4-13C]glutamate is incubated with yeast cells, the [13C]aspartate formed shows a [2-13C]/[3-13C] ratio that is greater than 1. If the conversion occurs through the Krebs TCA cycle with the symmetrical intermediates succinate and fumarate, the ratio is expected to be 1. The addition of fluoroacetate to the incubation increases the ratio further. When the mutants Aco1- or CS1- are used, little or no aspartate is formed. When [2-13C]acetate is incubated with yeast, the C2-/C3-aspartate ratio is 1.5 at 2 min and decreases to 1.0 after 10 min of incubation. These results indicate that orientation-conserved transfer occurs at the succinate thiokinase and succinate dehydrogenase steps of the Krebs TCA cycle.

UR - http://www.scopus.com/inward/record.url?scp=0027133418&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0027133418&partnerID=8YFLogxK

U2 - 10.1021/bi00210a022

DO - 10.1021/bi00210a022

M3 - Article

C2 - 8251492

VL - 32

SP - 12725

EP - 12729

JO - Biochemistry

JF - Biochemistry

SN - 0006-2960

IS - 47

ER -