Evidence of functional β-adrenoceptors in the cutaneous vasculature

C. G. Crandall, R. A. Etzel, J. M. Johnson

Research output: Contribution to journalArticle

49 Citations (Scopus)

Abstract

During a hyperthermic challenge, skin blood flow (SkBF) increases primarily through activation of the cutaneous active vasodilator system. However, mechanisms through which activation of this system elevates SkBF remain unknown. In this project, we sought to identify whether functional β-adrenoceptors exist on cutaneous vessels and, if present, whether these receptors play an important role in elevating SkBF during a hyperthermic challenge. In protocol 1, SkBF was assessed over two intradermal microdialysis probes. Initially, both probes were perfused with lactated Ringer solution. Probe A was then perfused with a 200 fiM solution of the β-adrenoceptor agonist isoproterenol while probe B was perfused with a 1.7 mM solution of the β-adrenoceptor antagonist propranolol. Isoproterenol perfusion significantly increased SkBF from 17.7 ±2.4 to 70.8 ± 13.2 perfusion units (PU; P < 0.05), whereas propranolol perfusion did not significantly affect SkBF (23.4 ±6.5 to 27.0 ±6.8 PU; P > 0.05). After this period, the solutions perfusing the probes were switched. Isoproterenol did not significantly change SkBF at the propranolol-treated site (27.0 ± 6.8 to 26.4 ±7.5 PU; P > 0.05). In protocol 2, SkBF was assessed over two microdialysis probes during indirect whole body heating. One probe was perfused with Ringer solution while the other probe was perfused with 1.7 mM propranolol. The degree of elevation in SkBF during heat stress at the propranolol-treated site (10.4 ±1.5 to 35.8 ±3.1 PU) was similar to the elevation in SkBF at the Ringer solution site (11.6 ±1.0 to 35.0 ±1.2 PU). These data demonstrate the presence of functional β-adrenoceptors in the skin; however, these receptors play no significant role in mediating cutaneous vasodilation during indirect whole body heating.

Original languageEnglish (US)
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume42
Issue number2
StatePublished - 1997

Fingerprint

Adrenergic Receptors
Skin
Propranolol
Isoproterenol
Microdialysis
Heating
Perfusion
Vasodilator Agents
Vasodilation
Hot Temperature

Keywords

  • Intradermal microdialysis
  • Isoproterenol
  • Laser-Doppler flowmetry
  • Laser-Doppler imaging
  • Propranolol

ASJC Scopus subject areas

  • Physiology

Cite this

Evidence of functional β-adrenoceptors in the cutaneous vasculature. / Crandall, C. G.; Etzel, R. A.; Johnson, J. M.

In: American Journal of Physiology - Heart and Circulatory Physiology, Vol. 42, No. 2, 1997.

Research output: Contribution to journalArticle

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