TY - JOUR
T1 - Evidence that the Igκ gene MAR regulates the probability of premature V-J joining and somatic hypermutation
AU - Yi, Ming
AU - Wu, Peiqing
AU - Trevorrow, Kenneth W.
AU - Claflin, Latham
AU - Garrard, William T.
PY - 1999/5/15
Y1 - 1999/5/15
N2 - The Igκ gene contains an evolutionarily conserved nuclear matrix association region (MAR) adjacent to the intronic enhancer. To test for the function of this MAR, we created mouse lines with a targeted MAR deletion. In MAR knockout animals, the immune system was normal in nearly all respects, including the distributions of various B cell populations and Ab levels. However, in pro-B cells, enhanced rearrangement was noted on the MAR- allele in heterozygotes. In addition, the efficiencies for targeting and generating somatic mutations were reduced on MAR-deleted alleles. These results provide evidence for the MAR negatively regulating the probability of premature rearrangement and positively regulating the probability of somatic hypermutation.
AB - The Igκ gene contains an evolutionarily conserved nuclear matrix association region (MAR) adjacent to the intronic enhancer. To test for the function of this MAR, we created mouse lines with a targeted MAR deletion. In MAR knockout animals, the immune system was normal in nearly all respects, including the distributions of various B cell populations and Ab levels. However, in pro-B cells, enhanced rearrangement was noted on the MAR- allele in heterozygotes. In addition, the efficiencies for targeting and generating somatic mutations were reduced on MAR-deleted alleles. These results provide evidence for the MAR negatively regulating the probability of premature rearrangement and positively regulating the probability of somatic hypermutation.
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M3 - Article
C2 - 10229843
AN - SCOPUS:0033561793
SN - 0022-1767
VL - 162
SP - 6029
EP - 6039
JO - Journal of Immunology
JF - Journal of Immunology
IS - 10
ER -