Evidence that the Igκ gene MAR regulates the probability of premature V-J joining and somatic hypermutation

Ming Yi, Peiqing Wu, Kenneth W. Trevorrow, Latham Claflin, William T. Garrard

Research output: Contribution to journalArticle

30 Scopus citations

Abstract

The Igκ gene contains an evolutionarily conserved nuclear matrix association region (MAR) adjacent to the intronic enhancer. To test for the function of this MAR, we created mouse lines with a targeted MAR deletion. In MAR knockout animals, the immune system was normal in nearly all respects, including the distributions of various B cell populations and Ab levels. However, in pro-B cells, enhanced rearrangement was noted on the MAR- allele in heterozygotes. In addition, the efficiencies for targeting and generating somatic mutations were reduced on MAR-deleted alleles. These results provide evidence for the MAR negatively regulating the probability of premature rearrangement and positively regulating the probability of somatic hypermutation.

Original languageEnglish (US)
Pages (from-to)6029-6039
Number of pages11
JournalJournal of Immunology
Volume162
Issue number10
StatePublished - May 15 1999

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Yi, M., Wu, P., Trevorrow, K. W., Claflin, L., & Garrard, W. T. (1999). Evidence that the Igκ gene MAR regulates the probability of premature V-J joining and somatic hypermutation. Journal of Immunology, 162(10), 6029-6039.