Evolutionary conservation of complexins

From choanoflagellates to mice

Xiaofei Yang, Jimin Pei, Yea Jin Kaeser-Woo, Taulant Bacaj, Nick V. Grishin, Thomas C. Südhof

Research output: Contribution to journalArticle

12 Citations (Scopus)

Abstract

Complexins are synaptic SNARE complex-binding proteins that cooperate with synaptotagmins in activating Ca2+-stimulated, synaptotagmin-dependent synaptic vesicle exocytosis and in clamping spontaneous, synaptotagmin-independent synaptic vesicle exocytosis. Here, we show that complexin sequences are conserved in some non-metazoan unicellular organisms and in all metazoans, suggesting that complexins are a universal feature of metazoans that predate metazoan evolution. We show that complexin from Nematostella vectensis, a cnidarian sea anemone far separated from mammals in metazoan evolution, functionally replaces mouse complexins in activating Ca2+-triggered exocytosis, but is unable to clamp spontaneous exocytosis. Thus, the activating function of complexins is likely conserved throughout metazoan evolution.

Original languageEnglish (US)
Pages (from-to)1308-1317
Number of pages10
JournalEMBO Reports
Volume16
Issue number10
DOIs
StatePublished - Oct 1 2015

Fingerprint

Choanoflagellata
Synaptotagmins
Exocytosis
Conservation
Synaptic Vesicles
SNARE Proteins
Mammals
Clamping devices
Sea Anemones
Cnidaria
Prednisolone
Conserved Sequence
Carrier Proteins
Constriction

Keywords

  • evolution
  • membrane fusion
  • SNARE proteins
  • synapse
  • synaptotagmin

ASJC Scopus subject areas

  • Genetics
  • Molecular Biology
  • Biochemistry

Cite this

Yang, X., Pei, J., Kaeser-Woo, Y. J., Bacaj, T., Grishin, N. V., & Südhof, T. C. (2015). Evolutionary conservation of complexins: From choanoflagellates to mice. EMBO Reports, 16(10), 1308-1317. https://doi.org/10.15252/embr.201540305

Evolutionary conservation of complexins : From choanoflagellates to mice. / Yang, Xiaofei; Pei, Jimin; Kaeser-Woo, Yea Jin; Bacaj, Taulant; Grishin, Nick V.; Südhof, Thomas C.

In: EMBO Reports, Vol. 16, No. 10, 01.10.2015, p. 1308-1317.

Research output: Contribution to journalArticle

Yang, X, Pei, J, Kaeser-Woo, YJ, Bacaj, T, Grishin, NV & Südhof, TC 2015, 'Evolutionary conservation of complexins: From choanoflagellates to mice', EMBO Reports, vol. 16, no. 10, pp. 1308-1317. https://doi.org/10.15252/embr.201540305
Yang, Xiaofei ; Pei, Jimin ; Kaeser-Woo, Yea Jin ; Bacaj, Taulant ; Grishin, Nick V. ; Südhof, Thomas C. / Evolutionary conservation of complexins : From choanoflagellates to mice. In: EMBO Reports. 2015 ; Vol. 16, No. 10. pp. 1308-1317.
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