Excitatory and inhibitory local circuit input to the rat dorsal motor nucleus of the vagus originating from the nucleus tractus solitarius

Scott F. Davis, Andrei V. Derbenev, Kevin W. Williams, Nicholas R. Glatzer, Bret N. Smith

Research output: Contribution to journalArticle

71 Citations (Scopus)

Abstract

The nucleus tractus solitarius (NTS) and dorsal motor nucleus of the vagus nerve (DMV) constitute sensory and motor nuclei of the dorsal vagal complex, respectively. We used whole-cell patch-clamp recordings from DMV neurons in rat brain slices and three methods of stimulation (electrical, glutamate microdrop, glutamate photostimulation) to test the hypothesis that convergent excitatory and inhibitory inputs to DMV neurons originate from intact neurons in multiple NTS areas. Electrical stimulation of the NTS resulted in evoked excitatory and inhibitory postsynaptic currents (eEPSCs and eIPSCs) in DMV neurons. Stimulation of the dorsal NTS with glutamate microdrops, which selectively stimulates the soma and dendrites of intact neurons, resulted in 31% of DMV neurons receiving eEPSCs, 44% receiving eIPSCs, and 6% receiving convergent excitatory and inhibitory inputs. Glutamate photostimulation allowed selective activation of intact neurons in multiple, discrete areas of the NTS and resulted in 36% of DMV neurons receiving eEPSCs, 65% receiving eIPSCs and 20% receiving both inputs. Data obtained by stimulation of multiple NTS areas support the hypothesis that there are anatomically convergent inputs to DMV neurons originating from intact neurons within the NTS. These data support the hypothesis that there is transfer of convergent information from the NTS to the DMV, implying that significant sensory-motor processing occurs within the brainstem.

Original languageEnglish (US)
Pages (from-to)208-217
Number of pages10
JournalBrain Research
Volume1017
Issue number1-2
DOIs
StatePublished - Aug 13 2004

Fingerprint

Solitary Nucleus
Neurons
Glutamic Acid
Electric Stimulation
Inhibitory Postsynaptic Potentials
Vagus Nerve
Excitatory Postsynaptic Potentials
Carisoprodol
Dendrites
Brain Stem

Keywords

  • GABA
  • Glutamate photostimulation
  • Interactions between neurotransmitters
  • Neurotransmitters, modulators, transporters, receptors
  • Patch-clamp
  • Reflex
  • Synaptic current

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Excitatory and inhibitory local circuit input to the rat dorsal motor nucleus of the vagus originating from the nucleus tractus solitarius. / Davis, Scott F.; Derbenev, Andrei V.; Williams, Kevin W.; Glatzer, Nicholas R.; Smith, Bret N.

In: Brain Research, Vol. 1017, No. 1-2, 13.08.2004, p. 208-217.

Research output: Contribution to journalArticle

Davis, Scott F. ; Derbenev, Andrei V. ; Williams, Kevin W. ; Glatzer, Nicholas R. ; Smith, Bret N. / Excitatory and inhibitory local circuit input to the rat dorsal motor nucleus of the vagus originating from the nucleus tractus solitarius. In: Brain Research. 2004 ; Vol. 1017, No. 1-2. pp. 208-217.
@article{aeb3a7e9d99948d293800c1ff585d7c1,
title = "Excitatory and inhibitory local circuit input to the rat dorsal motor nucleus of the vagus originating from the nucleus tractus solitarius",
abstract = "The nucleus tractus solitarius (NTS) and dorsal motor nucleus of the vagus nerve (DMV) constitute sensory and motor nuclei of the dorsal vagal complex, respectively. We used whole-cell patch-clamp recordings from DMV neurons in rat brain slices and three methods of stimulation (electrical, glutamate microdrop, glutamate photostimulation) to test the hypothesis that convergent excitatory and inhibitory inputs to DMV neurons originate from intact neurons in multiple NTS areas. Electrical stimulation of the NTS resulted in evoked excitatory and inhibitory postsynaptic currents (eEPSCs and eIPSCs) in DMV neurons. Stimulation of the dorsal NTS with glutamate microdrops, which selectively stimulates the soma and dendrites of intact neurons, resulted in 31{\%} of DMV neurons receiving eEPSCs, 44{\%} receiving eIPSCs, and 6{\%} receiving convergent excitatory and inhibitory inputs. Glutamate photostimulation allowed selective activation of intact neurons in multiple, discrete areas of the NTS and resulted in 36{\%} of DMV neurons receiving eEPSCs, 65{\%} receiving eIPSCs and 20{\%} receiving both inputs. Data obtained by stimulation of multiple NTS areas support the hypothesis that there are anatomically convergent inputs to DMV neurons originating from intact neurons within the NTS. These data support the hypothesis that there is transfer of convergent information from the NTS to the DMV, implying that significant sensory-motor processing occurs within the brainstem.",
keywords = "GABA, Glutamate photostimulation, Interactions between neurotransmitters, Neurotransmitters, modulators, transporters, receptors, Patch-clamp, Reflex, Synaptic current",
author = "Davis, {Scott F.} and Derbenev, {Andrei V.} and Williams, {Kevin W.} and Glatzer, {Nicholas R.} and Smith, {Bret N.}",
year = "2004",
month = "8",
day = "13",
doi = "10.1016/j.brainres.2004.05.049",
language = "English (US)",
volume = "1017",
pages = "208--217",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - Excitatory and inhibitory local circuit input to the rat dorsal motor nucleus of the vagus originating from the nucleus tractus solitarius

AU - Davis, Scott F.

AU - Derbenev, Andrei V.

AU - Williams, Kevin W.

AU - Glatzer, Nicholas R.

AU - Smith, Bret N.

PY - 2004/8/13

Y1 - 2004/8/13

N2 - The nucleus tractus solitarius (NTS) and dorsal motor nucleus of the vagus nerve (DMV) constitute sensory and motor nuclei of the dorsal vagal complex, respectively. We used whole-cell patch-clamp recordings from DMV neurons in rat brain slices and three methods of stimulation (electrical, glutamate microdrop, glutamate photostimulation) to test the hypothesis that convergent excitatory and inhibitory inputs to DMV neurons originate from intact neurons in multiple NTS areas. Electrical stimulation of the NTS resulted in evoked excitatory and inhibitory postsynaptic currents (eEPSCs and eIPSCs) in DMV neurons. Stimulation of the dorsal NTS with glutamate microdrops, which selectively stimulates the soma and dendrites of intact neurons, resulted in 31% of DMV neurons receiving eEPSCs, 44% receiving eIPSCs, and 6% receiving convergent excitatory and inhibitory inputs. Glutamate photostimulation allowed selective activation of intact neurons in multiple, discrete areas of the NTS and resulted in 36% of DMV neurons receiving eEPSCs, 65% receiving eIPSCs and 20% receiving both inputs. Data obtained by stimulation of multiple NTS areas support the hypothesis that there are anatomically convergent inputs to DMV neurons originating from intact neurons within the NTS. These data support the hypothesis that there is transfer of convergent information from the NTS to the DMV, implying that significant sensory-motor processing occurs within the brainstem.

AB - The nucleus tractus solitarius (NTS) and dorsal motor nucleus of the vagus nerve (DMV) constitute sensory and motor nuclei of the dorsal vagal complex, respectively. We used whole-cell patch-clamp recordings from DMV neurons in rat brain slices and three methods of stimulation (electrical, glutamate microdrop, glutamate photostimulation) to test the hypothesis that convergent excitatory and inhibitory inputs to DMV neurons originate from intact neurons in multiple NTS areas. Electrical stimulation of the NTS resulted in evoked excitatory and inhibitory postsynaptic currents (eEPSCs and eIPSCs) in DMV neurons. Stimulation of the dorsal NTS with glutamate microdrops, which selectively stimulates the soma and dendrites of intact neurons, resulted in 31% of DMV neurons receiving eEPSCs, 44% receiving eIPSCs, and 6% receiving convergent excitatory and inhibitory inputs. Glutamate photostimulation allowed selective activation of intact neurons in multiple, discrete areas of the NTS and resulted in 36% of DMV neurons receiving eEPSCs, 65% receiving eIPSCs and 20% receiving both inputs. Data obtained by stimulation of multiple NTS areas support the hypothesis that there are anatomically convergent inputs to DMV neurons originating from intact neurons within the NTS. These data support the hypothesis that there is transfer of convergent information from the NTS to the DMV, implying that significant sensory-motor processing occurs within the brainstem.

KW - GABA

KW - Glutamate photostimulation

KW - Interactions between neurotransmitters

KW - Neurotransmitters, modulators, transporters, receptors

KW - Patch-clamp

KW - Reflex

KW - Synaptic current

UR - http://www.scopus.com/inward/record.url?scp=3142768942&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=3142768942&partnerID=8YFLogxK

U2 - 10.1016/j.brainres.2004.05.049

DO - 10.1016/j.brainres.2004.05.049

M3 - Article

VL - 1017

SP - 208

EP - 217

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1-2

ER -