Existence and participation of xanthine oxidase in reperfusion injury of ischemic rabbit myocardium

L. S. Terada, J. D. Rubinstein, E. J. Lesnefsky, L. D. Horwitz, J. A. Leff, J. E. Repine

Research output: Contribution to journalArticle

58 Scopus citations

Abstract

Using a highly specific assay that minimizes enzyme inactivation in vitro, we found that rabbit myocardial tissue contained low levels of xanthine oxidase (XO) and xanthine dehydrogenase (XD) activity that were effectively inhibited by pretreatment of hearts with allopurinol. In parallel, allopurinol treatment also improved ventricular developed pressure, peak systolic pressure, and coronary flow in isolated hearts subjected to 30 min of normothermic global ischemia and 30 min of reperfusion. Although function was protected by allopurinol treatment, creatine kinase (CK) release was not altered by allopurinol. Inhibition of myocardial XO with allopurinol did not increase myocardial ATP or phosphocreatine. In addition, allopurinol did not scavenge superoxide anion or hydrogen peroxide in vitro. The results support the possibility that relatively low amounts of XO activity, similar to levels reported in human myocardium, may contribute to cardiac ischemia-reperfusion injury.

Original languageEnglish (US)
Pages (from-to)H805-H810
JournalAmerican Journal of Physiology - Heart and Circulatory Physiology
Volume260
Issue number3 29-3
DOIs
StatePublished - 1991

Keywords

  • Allopurinol
  • Hydrogen peroxide
  • Superoxide

ASJC Scopus subject areas

  • Physiology
  • Cardiology and Cardiovascular Medicine
  • Physiology (medical)

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