Exo1: A new chemical inhibitor of the exocytic pathway

Yan Feng, Sidney Yu, Troy K R Lasell, Ashutosh P. Jadhav, Eric Macia, Pierre Chardin, Paul Melancon, Michael Roth, Timothy Mitchison, Tomas Kirchhausen

Research output: Contribution to journalArticle

103 Scopus citations

Abstract

A phenotypic screen was used to search for drug-like molecules that can interfere with specific steps in membrane traffic. 2-(4-Fluorobenzoylamino)-benzoic acid methyl ester (Exo1), identified in this screen, induces a rapid collapse of the Golgi to the endoplasmic reticulum, thus acutely inhibiting the traffic emanating from the endoplasmic reticulum. Like Brefeldin A (BFA), Exo1 induces the rapid release of ADP-ribosylation factor (ARF) 1 from Golgi membranes but has less effect on the organization of the trans-Golgi network. Our data indicate that Exo1 acts by a different mechanism from BFA. Unlike BFA, Exo1 does not induce the ADP-ribosylation of CtBP/Bars50 and does not interfere with the activity of guanine nucleotide exchange factors specific for Golgi-based ARFS. Thus, Exo1 allows the fatty acid exchange activity of Bars50 to be distinguished from ARF1 activity in the control of Golgi tubulation.

Original languageEnglish (US)
Pages (from-to)6469-6474
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number11
DOIs
StatePublished - May 27 2003

Keywords

  • ADP-ribosylation factor
  • Bars50
  • Endoplasmic reticulum (ER)
  • Golgi
  • Imaging-based screen

ASJC Scopus subject areas

  • General

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    Feng, Y., Yu, S., Lasell, T. K. R., Jadhav, A. P., Macia, E., Chardin, P., Melancon, P., Roth, M., Mitchison, T., & Kirchhausen, T. (2003). Exo1: A new chemical inhibitor of the exocytic pathway. Proceedings of the National Academy of Sciences of the United States of America, 100(11), 6469-6474. https://doi.org/10.1073/pnas.0631766100