Exo1: A new chemical inhibitor of the exocytic pathway

Yan Feng, Sidney Yu, Troy K R Lasell, Ashutosh P. Jadhav, Eric Macia, Pierre Chardin, Paul Melancon, Michael Roth, Timothy Mitchison, Tomas Kirchhausen

Research output: Contribution to journalArticle

102 Citations (Scopus)

Abstract

A phenotypic screen was used to search for drug-like molecules that can interfere with specific steps in membrane traffic. 2-(4-Fluorobenzoylamino)-benzoic acid methyl ester (Exo1), identified in this screen, induces a rapid collapse of the Golgi to the endoplasmic reticulum, thus acutely inhibiting the traffic emanating from the endoplasmic reticulum. Like Brefeldin A (BFA), Exo1 induces the rapid release of ADP-ribosylation factor (ARF) 1 from Golgi membranes but has less effect on the organization of the trans-Golgi network. Our data indicate that Exo1 acts by a different mechanism from BFA. Unlike BFA, Exo1 does not induce the ADP-ribosylation of CtBP/Bars50 and does not interfere with the activity of guanine nucleotide exchange factors specific for Golgi-based ARFS. Thus, Exo1 allows the fatty acid exchange activity of Bars50 to be distinguished from ARF1 activity in the control of Golgi tubulation.

Original languageEnglish (US)
Pages (from-to)6469-6474
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume100
Issue number11
DOIs
StatePublished - May 27 2003

Fingerprint

Brefeldin A
Endoplasmic Reticulum
ADP-Ribosylation Factor 1
trans-Golgi Network
Guanine Nucleotide Exchange Factors
Membranes
Adenosine Diphosphate
Fatty Acids
Pharmaceutical Preparations

Keywords

  • ADP-ribosylation factor
  • Bars50
  • Endoplasmic reticulum (ER)
  • Golgi
  • Imaging-based screen

ASJC Scopus subject areas

  • Genetics
  • General

Cite this

Feng, Y., Yu, S., Lasell, T. K. R., Jadhav, A. P., Macia, E., Chardin, P., ... Kirchhausen, T. (2003). Exo1: A new chemical inhibitor of the exocytic pathway. Proceedings of the National Academy of Sciences of the United States of America, 100(11), 6469-6474. https://doi.org/10.1073/pnas.0631766100

Exo1 : A new chemical inhibitor of the exocytic pathway. / Feng, Yan; Yu, Sidney; Lasell, Troy K R; Jadhav, Ashutosh P.; Macia, Eric; Chardin, Pierre; Melancon, Paul; Roth, Michael; Mitchison, Timothy; Kirchhausen, Tomas.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 100, No. 11, 27.05.2003, p. 6469-6474.

Research output: Contribution to journalArticle

Feng, Y, Yu, S, Lasell, TKR, Jadhav, AP, Macia, E, Chardin, P, Melancon, P, Roth, M, Mitchison, T & Kirchhausen, T 2003, 'Exo1: A new chemical inhibitor of the exocytic pathway', Proceedings of the National Academy of Sciences of the United States of America, vol. 100, no. 11, pp. 6469-6474. https://doi.org/10.1073/pnas.0631766100
Feng, Yan ; Yu, Sidney ; Lasell, Troy K R ; Jadhav, Ashutosh P. ; Macia, Eric ; Chardin, Pierre ; Melancon, Paul ; Roth, Michael ; Mitchison, Timothy ; Kirchhausen, Tomas. / Exo1 : A new chemical inhibitor of the exocytic pathway. In: Proceedings of the National Academy of Sciences of the United States of America. 2003 ; Vol. 100, No. 11. pp. 6469-6474.
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