Exon-trapping mediated by the human retrotransposon SVA

Dustin C. Hancks, Adam D. Ewing, Jesse E. Chen, Katsushi Tokunaga, Haig H. Kazazian

Research output: Contribution to journalArticle

65 Scopus citations

Abstract

Although most human retrotransposons are inactive, both inactive and active retrotransposons drive genome evolution and may influence transcription through various mechanisms. In humans, three retrotransposon families are still active, but one of these, SVA, remains mysterious. Here we report the identification of a new subfamily of SVA, which apparently formed after an alternative splicing event where the first exon of the MAST2 gene spliced into an intronic SVA and subsequently retrotransposed. Additional examples of SVA retrotransposing upstream exons due to splicing into SVA were also identified in other primate genomes. After molecular and computational experiments, we found a number of functional 3′ splice sites within many different transcribed SVAs across the human and chimpanzee genomes. Using a minigene splicing construct containing an SVA, we observed splicing in cell culture, along with SVA exonization events that introduced premature termination codons (PTCs). These data imply that an SVA residing within an intron in the same orientation as the gene may alter normal gene transcription either by gene-trapping or by introducing PTCs through exonization, possibly creating differences within and across species.

Original languageEnglish (US)
Pages (from-to)1983-1991
Number of pages9
JournalGenome Research
Volume19
Issue number11
DOIs
StatePublished - Nov 2009

ASJC Scopus subject areas

  • Genetics
  • Genetics(clinical)

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    Hancks, D. C., Ewing, A. D., Chen, J. E., Tokunaga, K., & Kazazian, H. H. (2009). Exon-trapping mediated by the human retrotransposon SVA. Genome Research, 19(11), 1983-1991. https://doi.org/10.1101/gr.093153.109