Expansion of CD27high plasmablasts in transverse myelitis patients that utilize VH4 and JH6 genes and undergo extensive somatic hypermutation

A. J. Ligocki, W. H. Rounds, E. M. Cameron, C. T. Harp, Elliot Frohman, A. M. Courtney, Steven Vernino, Lindsay G Cowell, Benjamin Greenberg, Nancy L Monson

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

Patients with the autoimmune disease multiple sclerosis (MS) typically present with the clinically isolated syndromes (CIS) transverse myelitis (TM) or optic neuritis (ON). B-cell disturbances have been well documented in patients with MS and CIS patients with ON, but not in CIS patients with TM, despite the fact that these patients have the worst clinical outcome of all CIS types. The goal of this study was to characterize the B-cell populations and immunoglobulin genetics in TM patients. We found a unique expansion of CD27high plasmablasts in both the cerebrospinal fluid and periphery of TM patients that is not present in ON patients. Additionally, plasmablasts from TM patients show evidence for positive selection with increased somatic hypermutation accumulation in VH4+ B cells and receptor editing that is not observed in ON patients. These characteristics unique to TM patients may impact disease severity and progression.

Original languageEnglish (US)
Pages (from-to)291-301
Number of pages11
JournalGenes and Immunity
Volume14
Issue number5
DOIs
StatePublished - Jul 2013

Fingerprint

Transverse Myelitis
Genes
Optic Neuritis
B-Lymphocytes
Multiple Sclerosis
Population Genetics
Autoimmune Diseases
Cerebrospinal Fluid
Disease Progression
Immunoglobulins

Keywords

  • Antibody repertoire
  • Clinically isolated syndrome
  • Multiple sclerosis
  • Optic neuritis
  • Plasmablast
  • Transverse myelitis

ASJC Scopus subject areas

  • Genetics(clinical)
  • Immunology
  • Genetics
  • Medicine(all)

Cite this

Expansion of CD27high plasmablasts in transverse myelitis patients that utilize VH4 and JH6 genes and undergo extensive somatic hypermutation. / Ligocki, A. J.; Rounds, W. H.; Cameron, E. M.; Harp, C. T.; Frohman, Elliot; Courtney, A. M.; Vernino, Steven; Cowell, Lindsay G; Greenberg, Benjamin; Monson, Nancy L.

In: Genes and Immunity, Vol. 14, No. 5, 07.2013, p. 291-301.

Research output: Contribution to journalArticle

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abstract = "Patients with the autoimmune disease multiple sclerosis (MS) typically present with the clinically isolated syndromes (CIS) transverse myelitis (TM) or optic neuritis (ON). B-cell disturbances have been well documented in patients with MS and CIS patients with ON, but not in CIS patients with TM, despite the fact that these patients have the worst clinical outcome of all CIS types. The goal of this study was to characterize the B-cell populations and immunoglobulin genetics in TM patients. We found a unique expansion of CD27high plasmablasts in both the cerebrospinal fluid and periphery of TM patients that is not present in ON patients. Additionally, plasmablasts from TM patients show evidence for positive selection with increased somatic hypermutation accumulation in VH4+ B cells and receptor editing that is not observed in ON patients. These characteristics unique to TM patients may impact disease severity and progression.",
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