Experimental approaches to the study of reversible protein fatty acylation in mammalian cells

Guy James, Eric Olson

Research output: Contribution to journalArticlepeer-review

7 Scopus citations

Abstract

Recent studies suggest a role for reversible fatty acylation of proteins in diverse cellular processes, ranging from modulation of the plasma membrane-cytoskeleton interface to signal transduction, and perhaps even vesicular transport. In this article, we review current evidence relevant to this topic and present experimental methods useful in the study of dynamic fatty acylation. Included are protocols for metabolic labeling with fatty acids, pulse-chase experiments, linkage analyses, and chemical identification of fatty acids, along with discussions of possible problems that might be encountered.

Original languageEnglish (US)
Pages (from-to)270-275
Number of pages6
JournalMethods
Volume1
Issue number3
DOIs
StatePublished - Dec 1990

ASJC Scopus subject areas

  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)

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