TY - JOUR
T1 - Experimental approaches to the study of reversible protein fatty acylation in mammalian cells
AU - James, Guy
AU - Olson, Eric
N1 - Funding Information:
Work in the authors' laboratory is supported by grants from ACS and NIH. E.N.O. is an Established Investigator of the American Heart Association.
PY - 1990/12
Y1 - 1990/12
N2 - Recent studies suggest a role for reversible fatty acylation of proteins in diverse cellular processes, ranging from modulation of the plasma membrane-cytoskeleton interface to signal transduction, and perhaps even vesicular transport. In this article, we review current evidence relevant to this topic and present experimental methods useful in the study of dynamic fatty acylation. Included are protocols for metabolic labeling with fatty acids, pulse-chase experiments, linkage analyses, and chemical identification of fatty acids, along with discussions of possible problems that might be encountered.
AB - Recent studies suggest a role for reversible fatty acylation of proteins in diverse cellular processes, ranging from modulation of the plasma membrane-cytoskeleton interface to signal transduction, and perhaps even vesicular transport. In this article, we review current evidence relevant to this topic and present experimental methods useful in the study of dynamic fatty acylation. Included are protocols for metabolic labeling with fatty acids, pulse-chase experiments, linkage analyses, and chemical identification of fatty acids, along with discussions of possible problems that might be encountered.
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U2 - 10.1016/S1046-2023(05)80327-5
DO - 10.1016/S1046-2023(05)80327-5
M3 - Article
AN - SCOPUS:38249016955
SN - 1046-2023
VL - 1
SP - 270
EP - 275
JO - Methods
JF - Methods
IS - 3
ER -