Recent studies suggest a role for reversible fatty acylation of proteins in diverse cellular processes, ranging from modulation of the plasma membrane-cytoskeleton interface to signal transduction, and perhaps even vesicular transport. In this article, we review current evidence relevant to this topic and present experimental methods useful in the study of dynamic fatty acylation. Included are protocols for metabolic labeling with fatty acids, pulse-chase experiments, linkage analyses, and chemical identification of fatty acids, along with discussions of possible problems that might be encountered.
ASJC Scopus subject areas
- Molecular Biology
- Biochemistry, Genetics and Molecular Biology(all)