Experimental elongation of telomeres extends the lifespan of immortal x normal cell hybrids

Woodring E. Wright, Dana Brašiškyte̊, Mieczyslaw A. Piatyszek, Jerry W. Shay

Research output: Contribution to journalArticle

135 Scopus citations

Abstract

Hybrids between immortal cells that express telomerase and normal cells that lack telomerase have a limited lifespan. We demonstrate that telomerase is repressed in such hybrids. Treatment of immortal human cell lines with certain oligonucleotides resulted in telomere elongation. We took advantage of this observation to test the hypothesis that elongation of telomeres would extend the lifespan of cells in culture. An immortal human cell line was treated with an oligonucleotide to lengthen its telomeres and then was fused with mortal cells. The lifespan of these hybrid cells was longer than that of the hybrids in which telomeres had not been elongated. These observations provide the first direct evidence supporting the hypothesis that telomere length determines proliferative capacity of human cells.

Original languageEnglish (US)
Pages (from-to)1734-1741
Number of pages8
JournalEMBO Journal
Volume15
Issue number7
DOIs
StatePublished - Apr 1 1996

Keywords

  • Aging
  • Cancer
  • Cell hybrids
  • Telomerase
  • Telomeres

ASJC Scopus subject areas

  • Neuroscience(all)
  • Molecular Biology
  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)

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