Experimental elongation of telomeres extends the lifespan of immortal x normal cell hybrids

Woodring E. Wright, Dana Brašiškyte̊, Mieczyslaw A. Piatyszek, Jerry W. Shay

Research output: Contribution to journalArticle

134 Citations (Scopus)

Abstract

Hybrids between immortal cells that express telomerase and normal cells that lack telomerase have a limited lifespan. We demonstrate that telomerase is repressed in such hybrids. Treatment of immortal human cell lines with certain oligonucleotides resulted in telomere elongation. We took advantage of this observation to test the hypothesis that elongation of telomeres would extend the lifespan of cells in culture. An immortal human cell line was treated with an oligonucleotide to lengthen its telomeres and then was fused with mortal cells. The lifespan of these hybrid cells was longer than that of the hybrids in which telomeres had not been elongated. These observations provide the first direct evidence supporting the hypothesis that telomere length determines proliferative capacity of human cells.

Original languageEnglish (US)
Pages (from-to)1734-1741
Number of pages8
JournalEMBO Journal
Volume15
Issue number7
StatePublished - Apr 1 1996

Fingerprint

Hybrid Cells
Telomerase
Telomere
Elongation
Cells
Oligonucleotides
Cell culture
Cell Line
Cell Culture Techniques

Keywords

  • Aging
  • Cancer
  • Cell hybrids
  • Telomerase
  • Telomeres

ASJC Scopus subject areas

  • Genetics
  • Cell Biology

Cite this

Wright, W. E., Brašiškyte̊, D., Piatyszek, M. A., & Shay, J. W. (1996). Experimental elongation of telomeres extends the lifespan of immortal x normal cell hybrids. EMBO Journal, 15(7), 1734-1741.

Experimental elongation of telomeres extends the lifespan of immortal x normal cell hybrids. / Wright, Woodring E.; Brašiškyte̊, Dana; Piatyszek, Mieczyslaw A.; Shay, Jerry W.

In: EMBO Journal, Vol. 15, No. 7, 01.04.1996, p. 1734-1741.

Research output: Contribution to journalArticle

Wright, WE, Brašiškyte̊, D, Piatyszek, MA & Shay, JW 1996, 'Experimental elongation of telomeres extends the lifespan of immortal x normal cell hybrids', EMBO Journal, vol. 15, no. 7, pp. 1734-1741.
Wright, Woodring E. ; Brašiškyte̊, Dana ; Piatyszek, Mieczyslaw A. ; Shay, Jerry W. / Experimental elongation of telomeres extends the lifespan of immortal x normal cell hybrids. In: EMBO Journal. 1996 ; Vol. 15, No. 7. pp. 1734-1741.
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